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Description:
Proliferating cell nuclear antigen (PCNA) (Cyclin) (Mutagen-sensitive209 protein).
Molecular weight: 28830
View which proteins in this organism that is involved with DNA Repair;
classified after biological processes (using data from the GOA project):
nucleotide-excision repair (and GO:0045001, a synonym)( GO:0006289 ) mismatch repair( GO:0006298 )
Important dates:
01-NOV-1990, integrated into UniProtKB/Swiss-Prot.
01-FEB-1991, sequence version 2.
07-FEB-2006, entry version 59.
Phylogenetic order:
Eukaryota Metazoa Arthropoda Hexapoda Insecta Pterygota Neoptera Endopterygota Diptera Brachycera Muscomorpha Ephydroidea Drosophilidae Drosophila.
To calculate the pI (Isoelectric point - the pH where a protein has a neutral charge),
go to this page and enter the protein ID (e.g 3MG_ECOLI): http://us.expasy.org/tools/pi_tool.html
Links to references in other databases for protein PCNA_DROME:
| Database | Pointer | Add. info#1 | Add. info#2 |
| EMBL | M33950 | AAA28746.1 | - |
| EMBL | AE003792 | AAF57493.1 | - |
| PIR | A34752 | A34752. | |
| HSSP | P12004 | 1AXC | |
| SMR | P17917 | 1-259.1 | |
| IntAct | P17917 | -.1 | |
| Ensembl | CG9193 | Drosophila melanogaster.1 | |
| FlyBase | FBgn0005655 | mus209. | |
| BioCyc | DMEL-XXX-02:DMEL-XXX-02-005619-MONOMER | -.1 | |
| GO | GO:0005515 | F:protein binding | IPI. |
| GO | GO:0007307 | P:chorion gene amplification | TAS. |
| GO | GO:0006261 | P:DNA-dependent DNA replication | TAS. |
| GO | GO:0006298 | P:mismatch repair | TAS. |
| GO | GO:0007052 | P:mitotic spindle organization and biogenesis | IMP. |
| GO | GO:0006289 | P:nucleotide-excision repair | TAS. |
| InterPro | IPR000730 | Pr_cel_nuc_antig. | |
| PANTHER | PTHR11352 | Pr_cel_nuc_antig.1 | 1. |
| Pfam | PF02747 | PCNA_C | 1. |
| Pfam | PF00705 | PCNA_N | 1. |
| PIRSF | PIRSF002090 | PCNA | 1. |
| PRINTS | PR00339 | PCNACYCLIN. | |
| ProDom | PD002673 | Pr_cel_nuc_antig | 1. |
| TIGRFAMs | TIGR00590 | pcna | 1. |
| PROSITE | PS01251 | PCNA_1 | 1. |
| PROSITE | PS00293 | PCNA_2 | 1. |
Keywords:
Complete proteome; Direct protein sequencing; DNA replication; DNA-binding; Nuclear protein.
References:
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX MEDLINE=90158601; PubMed=1968224;
RA Yamaguchi M., Nishida Y., Moriuchi T., Hirose F., Hui C.C., Suzuki Y.,
RA Matsukage A.;
RT "Drosophila proliferating cell nuclear antigen (cyclin) gene:
RT structure, expression during development, and specific binding of
RT homeodomain proteins to its 5'-flanking region.";
RL Mol. Cell. Biol. 10:872-879(1990).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX MEDLINE=20196006; PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X.,
RA Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D.,
RA Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G.,
RA Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D.,
RA Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M.,
RA Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S.,
RA Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P.,
RA Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I.,
RA Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P.,
RA de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M.,
RA Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P.,
RA Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W.,
RA Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K.,
RA Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J.,
RA Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C.,
RA Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A.,
RA Kimmel B.E., Kodira C.D., Kraft C., Kravitz S., Kulp D., Lai Z.,
RA Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X.,
RA Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D.,
RA Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A.,
RA Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L.,
RA Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M.,
RA Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G.,
RA Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H.,
RA Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E.,
RA Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X.,
RA Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J.,
RA Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A.,
RA Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L.,
RA Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S., Zhu X., Smith H.O.,
RA Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [3]
RP GENOME REANNOTATION.
RX MEDLINE=22426069; PubMed=12537572;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q.,
RA Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M.,
RA Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a
RT systematic review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [4]
RP PROTEIN SEQUENCE OF 1-22.
RC STRAIN=Oregon-R;
RX MEDLINE=90307726; PubMed=1973166;
RA Ng L., Prelich G., Anderson C.W., Stillman B., Fisher P.A.;
RT "Drosophila proliferating cell nuclear antigen. Structural and
RT functional homology with its mammalian counterpart.";
RL J. Biol. Chem. 265:11948-11954(1990).
RN [5]
RP MUTAGENESIS.
RX MEDLINE=94185652; PubMed=7907981;
RA Henderson D.S., Banga S.S., Grigliatto T.A., Boyd J.B.;
RT "Mutagen sensitivity and suppression of position-effect variegation
RT result from mutations in mus209, the Drosophila gene encoding PCNA.";
RL EMBO J. 13:1450-1459(1994).
Feature:
CHAIN 1 260 Proliferating cell nuclear antigen.
/FTId=PRO_0000149170.
DNA_BIND 61 80 Potential.
Comments:
-!- FUNCTION: This protein is an auxiliary protein of DNA polymerase
delta and is involved in the control of eukaryotic DNA replication
by increasing the polymerase's processibility during elongation of
the leading strand.
-!- SUBUNIT: Homotrimer. Forms a complex with activator 1
heteropentamer in the presence of ATP (By similarity).
-!- INTERACTION:
Q8IQI1:CG17666; NbExp=1; IntAct=EBI-148806, EBI-139867;
Q9VE69:CG31122; NbExp=1; IntAct=EBI-148806, EBI-94009;
Q94536:dap; NbExp=1; IntAct=EBI-148806, EBI-89179;
-!- SUBCELLULAR LOCATION: Nucleus.
-!- SIMILARITY: Belongs to the PCNA family.
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Sequence length: 260
MFEARLGQAT ILKKILDAIK DLLNEATFDC SDSGIQLQAM DNSHVSLVSL TLRSDGFDKF
RCDRNLSMGM NLGSMAKILK CANNEDNVTM KAQDNADTVT IMFESANQEK VSDYEMKLMN
LDQEHLGIPE TDFSCVVRMP AMEFARICRD LAQFSESVVI CCTKEGVKFS ASGDVGTANI
KLAQTGSVDK EEEAVIIEMQ EPVTLTFACR YLNAFTKATP LSTQVQLSMC ADVPLVVEYA
IKDLGHIRYY LAPKIEDNET