Description:
Exodeoxyribonuclease III (EC 3.1.11.2) (Exonuclease III) (EXO III).
Molecular weight: 31025
View which proteins in this organism that is involved with DNA Repair;
classified after biological processes (using data from the GOA project):
DNA repair( GO:0006281 )
Important dates:
01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
01-NOV-1995, sequence version 1.
07-MAR-2006, entry version 49.
Phylogenetic order:
Bacteria Proteobacteria Gammaproteobacteria Pasteurellales Pasteurellaceae Haemophilus.
To calculate the pI (Isoelectric point - the pH where a protein has a neutral charge),
go to this page and enter the protein ID (e.g 3MG_ECOLI): http://us.expasy.org/tools/pi_tool.html
Links to references in other databases for protein EX3_HAEIN:
| Database | Pointer | Add. info#1 | Add. info#2 |
| EMBL | L42023 | AAC21719.1 | - |
| PIR | H64044 | H64044. | |
| HSSP | P09030 | 1AKO | |
| SMR | P44318 | 1-267.1 | |
| GenomeReviews | L42023_GR | HI0041.1 | |
| TIGR | HI0041 | -. | |
| BioCyc | HINF71421:HI0041-MONOMER | -.1 | |
| InterPro | IPR000097 | APendonclse1. | |
| InterPro | IPR005135 | Exo_endo_phos. | |
| InterPro | IPR004808 | ExoIII_xth. | |
| Pfam | PF03372 | Exo_endo_phos | 1. |
| TIGRFAMs | TIGR00195 | exoDNase_III | 1. |
| TIGRFAMs | TIGR00633 | xth | 1. |
| PROSITE | PS00726 | AP_NUCLEASE_F1_1 | 1. |
| PROSITE | PS00727 | AP_NUCLEASE_F1_2 | 1. |
| PROSITE | PS00728 | AP_NUCLEASE_F1_3 | 1. |
Keywords:
Complete proteome; DNA damage; DNA repair; Exonuclease; Hydrolase; Magnesium; Metal-binding; Nuclease.
References:
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Rd / KW20 / ATCC 51907;
RX MEDLINE=95350630; PubMed=7542800;
RA Fleischmann R.D., Adams M.D., White O., Clayton R.A., Kirkness E.F.,
RA Kerlavage A.R., Bult C.J., Tomb J.-F., Dougherty B.A., Merrick J.M.,
RA McKenney K., Sutton G.G., FitzHugh W., Fields C.A., Gocayne J.D.,
RA Scott J.D., Shirley R., Liu L.-I., Glodek A., Kelley J.M.,
RA Weidman J.F., Phillips C.A., Spriggs T., Hedblom E., Cotton M.D.,
RA Utterback T.R., Hanna M.C., Nguyen D.T., Saudek D.M., Brandon R.C.,
RA Fine L.D., Fritchman J.L., Fuhrmann J.L., Geoghagen N.S.M.,
RA Gnehm C.L., McDonald L.A., Small K.V., Fraser C.M., Smith H.O.,
RA Venter J.C.;
RT "Whole-genome random sequencing and assembly of Haemophilus influenzae
RT Rd.";
RL Science 269:496-512(1995).
Feature:
CHAIN 1 267 Exodeoxyribonuclease III.
/FTId=PRO_0000200022.
ACT_SITE 259 259 Proton acceptor (By similarity).
METAL 34 34 Magnesium or manganese (By similarity).
Comments:
-!- FUNCTION: Major apurinic-apyrimidinic endonuclease of E.coli. It
removes the damaged DNA at cytosines and guanines by cleaving on
the 3' side of the AP site by a beta-elimination reaction (By
similarity).
-!- CATALYTIC ACTIVITY: Exonucleolytic cleavage in the 3'- to 5'-
direction to yield nucleoside 5'-phosphates.
-!- SUBUNIT: Monomer (By similarity).
-!- SIMILARITY: Belongs to the DNA repair enzymes AP/exoA family.
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Sequence length: 267
MKFISFNING LRARPHQLEA IIEKYQPDVI GLQEIKVADE AFPYEITENL GYHVFHHGQK
GHYGVALLTK QEPKVIRRGF PTDNEDAQKR IIMADLETEF GLLTVINGYF PQGESRAHET
KFPAKEKFYA DLQQYLEKEH DKSNPILIMG DMNISPSDLD IGIGDENRKR WLRTGKCSFL
PEERAWYQRL YDYGLEDSFR KLNPTANDKF SWFDYRSKGF DDNRGLRIDH ILVSQKLAER
CVDVGIALDI RAMEKPSDHA PIWAEFK