Description:
UvrABC system protein B (Protein uvrB) (Excinuclease ABC subunit B).
Molecular weight: 76986
View which proteins in this organism that is involved with DNA Repair;
classified after biological processes (using data from the GOA project):
DNA repair( GO:0006281 ) base-excision repair( GO:0006284 ) nucleotide-excision repair (and GO:0045001, a synonym)( GO:0006289 )
Important dates:
13-AUG-2002, integrated into UniProtKB/Swiss-Prot.
01-JUN-2002, sequence version 1.
07-MAR-2006, entry version 27.
Phylogenetic order:
Bacteria Fusobacteria Fusobacterales Fusobacteriaceae Fusobacterium.
To calculate the pI (Isoelectric point - the pH where a protein has a neutral charge),
go to this page and enter the protein ID (e.g 3MG_ECOLI): http://us.expasy.org/tools/pi_tool.html
Links to references in other databases for protein UVRB_FUSNN:
| Database | Pointer | Add. info#1 | Add. info#2 |
| EMBL | AE009951 | AAL94430.1 | - |
| HSSP | P56981 | 1D9X | |
| GenomeReviews | AE009951_GR | FN0224.1 | |
| BioCyc | FNUC190304:FN0224-MONOMER | -.1 | |
| HAMAP | MF_00204 | - | 1. |
| InterPro | IPR001410 | DEAD. | |
| InterPro | IPR011545 | DEAD/DEAH_N. | |
| InterPro | IPR001650 | Helicase_C. | |
| InterPro | IPR006935 | ResIII. | |
| InterPro | IPR001943 | UvrB/C. | |
| InterPro | IPR004807 | UvrB_ABC. | |
| Pfam | PF00271 | Helicase_C | 1. |
| Pfam | PF04851 | ResIII | 1. |
| Pfam | PF02151 | UVR | 1. |
| SMART | SM00487 | DEXDc | 1. |
| SMART | SM00490 | HELICc | 1. |
| TIGRFAMs | TIGR00631 | uvrb | 1. |
| PROSITE | PS50151 | UVR | 1. |
Keywords:
ATP-binding; Complete proteome; DNA damage; DNA excision; DNA repair; Excision nuclease; Nucleotide-binding; SOS response.
References:
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 25586;
RX MEDLINE=21886394; PubMed=11889109;
RX DOI=10.1128/JB.184.7.2005-2018.2002;
RA Kapatral V., Anderson I., Ivanova N., Reznik G., Los T., Lykidis A.,
RA Bhattacharyya A., Bartman A., Gardner W., Grechkin G., Zhu L.,
RA Vasieva O., Chu L., Kogan Y., Chaga O., Goltsman E., Bernal A.,
RA Larsen N., D'Souza M., Walunas T., Pusch G., Haselkorn R.,
RA Fonstein M., Kyrpides N.C., Overbeek R.;
RT "Genome sequence and analysis of the oral bacterium Fusobacterium
RT nucleatum strain ATCC 25586.";
RL J. Bacteriol. 184:2005-2018(2002).
Feature:
CHAIN 1 663 UvrABC system protein B.
/FTId=PRO_0000138393.
DOMAIN 624 659 UVR.
NP_BIND 40 47 ATP (Potential).
MOTIF 93 116 Beta-hairpin.
Comments:
-!- FUNCTION: The UvrABC repair system catalyzes the recognition and
processing of DNA lesions. A damage recognition complex composed
of 2 uvrA and 2 uvrB subunits scans DNA for abnormalities. Upon
binding of the uvrA(2)B(2) complex to a putative damaged site, the
DNA wraps around one uvrB monomer. DNA wrap is dependent on ATP
binding by uvrB and probably causes local melting of the DNA
helix, facilitating insertion of uvrB beta-hairpin between the DNA
strands. Then uvrB probes one DNA strand for the presence of a
lesion. If a lesion is found the uvrA subunits dissociate and the
uvrB-DNA preincision complex is formed. This complex is
subsequently bound by uvrC and the second uvrB is released. If no
lesion is found, the DNA wraps around the other uvrB subunit that
will check the other stand for damage (By similarity).
-!- SUBUNIT: Forms a heterotetramer with uvrA during the search for
lesions. Interacts with uvrC in an incision complex (By
similarity).
-!- SUBCELLULAR LOCATION: Cytoplasm (By similarity).
-!- DOMAIN: The beta-hairpin motif is involved in DNA binding (By
similarity).
-!- SIMILARITY: Belongs to the uvrB family.
-!- SIMILARITY: Contains 1 UVR domain.
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Sequence length: 663
MENNLFKIHS DYKPTGDQPT AIDSIVKNIE NGVKDQVLLG VTGSGKTFTI ANVIERVQRP
SLIIAPNKTL AAQLYSEYKK FFPENAVEYF VSYYDYYQPE AYIKTTDTYI EKDSSVNDEI
DKLRNAATAA LIHRRDVIIV ASVSSIYGLG SPDTYRRMTI PIDKQTGISR KELMKRLIAL
RYDRNDVAFE RGQFRIKGDV IDIYPSYMNN GYRLEYWGDD LEEISEINTL TGQKVKKNLE
RIVIYPATQY LTADDDKDRI IQEIKDDLKV EVKKFEDDKK LLEAQRLRQR TEYDLEMITE
IGYCKGIENY SRYLAGKNPG DTPDTLFEYF PKDFLLFIDE SHITVPQVRG MYNGDRARKE
SLVENGFRLK AALDNRPLRF EEFREKSNQT VFISATPGDF EVEVSDNHIA EQLIRPTGIV
DPEIEIRPTK NQVDDLLDEI RKRAAKKERV LVTTLTKKIA EELTEYYIEL GVKVKYMHSD
IDTLERIEII RALRKGEIDV IIGINLLREG LDIPEVSLVA IMEADKEGFL RSRRSLVQTI
GRAARNVEGR VILYADIMTD SMKEAITETE RRRKIQKEYN AYNHIDPKSI IKEIAEDLIN
LDYGIEEKKF ENDKKVFRNK TDIEKEITKL EKKIKKLVEE LDFEQAIILR DEMLKLKELL
LEF