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Description:
Crossover junction endodeoxyribonuclease ruvC (EC 3.1.22.4) (Hollidayjunction nuclease ruvC) (Holliday juction resolvase ruvC).
Molecular weight: 17262
View which proteins in this organism that is involved with DNA Repair;
classified after biological processes (using data from the GOA project):
DNA repair( GO:0006281 )
Important dates:
31-JAN-2002, integrated into UniProtKB/Swiss-Prot.
01-DEC-2001, sequence version 1.
07-MAR-2006, entry version 21.
Phylogenetic order:
Bacteria Proteobacteria Alphaproteobacteria Rickettsiales Rickettsiaceae Rickettsieae Rickettsia spotted fever group.
To calculate the pI (Isoelectric point - the pH where a protein has a neutral charge),
go to this page and enter the protein ID (e.g 3MG_ECOLI): http://us.expasy.org/tools/pi_tool.html
Links to references in other databases for protein RUVC_RICCN:
| Database | Pointer | Add. info#1 | Add. info#2 |
| EMBL | AE008582 | AAL02697.1 | - |
| PIR | G97719 | G97719. | |
| HSSP | P24239 | 1HJR | |
| GenomeReviews | AE006914_GR | RC0159.1 | |
| BioCyc | RCON781:RC0159-MONOMER | -.1 | |
| HAMAP | MF_00034 | - | 1. |
| InterPro | IPR012337 | RNaseH_fold. | |
| InterPro | IPR002176 | RuvC. | |
| Pfam | PF02075 | RuvC | 1. |
| PRINTS | PR00696 | RSOLVASERUVC. | |
| ProDom | PD008333 | RuvC | 1. |
| TIGRFAMs | TIGR00228 | ruvC | 1. |
| PROSITE | PS01321 | RUVC | FALSE_NEG. |
Keywords:
Complete proteome; DNA damage; DNA recombination; DNA repair; Hydrolase; Magnesium; Metal-binding; Nuclease.
References:
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Malish 7;
RX MEDLINE=21442074; PubMed=11557893; DOI=10.1126/science.1061471;
RA Ogata H., Audic S., Renesto-Audiffren P., Fournier P.-E., Barbe V.,
RA Samson D., Roux V., Cossart P., Weissenbach J., Claverie J.-M.,
RA Raoult D.;
RT "Mechanisms of evolution in Rickettsia conorii and R. prowazekii.";
RL Science 293:2093-2098(2001).
Feature:
CHAIN 1 157 Crossover junction endodeoxyribonuclease
ruvC.
/FTId=PRO_0000183128.
METAL 7 7 Magnesium (By similarity).
METAL 67 67 Magnesium (By similarity).
METAL 140 140 Magnesium (By similarity).
METAL 143 143 Magnesium (By similarity).
Comments:
-!- FUNCTION: Nuclease that resolves Holliday junction intermediates
in genetic recombination. Cleaves the cruciform structure in
supercoiled DNA by nicking to strands with the same polarity at
sites symmetrically opposed at the junction in the homologous arms
and leaves a 5'terminal phosphate and a 3'terminal hydroxyl group
(By similarity).
-!- CATALYTIC ACTIVITY: Endonucleolytic cleavage at a junction such as
a reciprocal single-stranded crossover between two homologous DNA
duplexes (Holliday junction).
-!- COFACTOR: Binds 1 magnesium ion per subunit (By similarity).
-!- SIMILARITY: Belongs to the ruvC family.
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Sequence length: 157
MIILGIDPAL GSLGWAVVAK ETAQLKYLAS GIIRTNSKDA IHHRLAFINS TLEKVILEYQ
PNMVAIEETF VNTNSVTSLK LGYARGAIMS LIGRYNLDMR EFKPNTVKKT VTGYGHAEKD
QMLHMIKLLL SGTALITNSD EADAVAIAYT CLVTKNY