Description:
UvrABC system protein B (Protein uvrB) (Excinuclease ABC subunit B).
Molecular weight: 76095
View which proteins in this organism that is involved with DNA Repair;
classified after biological processes (using data from the GOA project):
DNA repair( GO:0006281 ) base-excision repair( GO:0006284 ) nucleotide-excision repair (and GO:0045001, a synonym)( GO:0006289 )
Important dates:
01-APR-1988, integrated into UniProtKB/Swiss-Prot.
07-JUN-2005, sequence version 1.
07-MAR-2006, entry version 10.
Phylogenetic order:
Bacteria Proteobacteria Gammaproteobacteria Enterobacteriales Enterobacteriaceae Escherichia.
To calculate the pI (Isoelectric point - the pH where a protein has a neutral charge),
go to this page and enter the protein ID (e.g 3MG_ECOLI): http://us.expasy.org/tools/pi_tool.html
Links to references in other databases for protein UVRB_ECO57:
| Database | Pointer | Add. info#1 | Add. info#2 |
| EMBL | AE005174 | AAG55150.1 | - |
| EMBL | BA000007 | BAB34280.1 | - |
| PIR | A99736 | A99736. | |
| PIR | B85586 | B85586. | |
| SMR | P0A8F9 | 617-672.1 | |
| GenomeReviews | BA000007_GR | ECs0857.1 | |
| GenomeReviews | AE005174_GR | z0998.1 | |
| HAMAP | MF_00204 | - | 1. |
| InterPro | IPR001410 | DEAD. | |
| InterPro | IPR011545 | DEAD/DEAH_N. | |
| InterPro | IPR001650 | Helicase_C. | |
| InterPro | IPR001943 | UvrB/C. | |
| InterPro | IPR004807 | UvrB_ABC. | |
| Pfam | PF00271 | Helicase_C | 1. |
| Pfam | PF04851 | ResIII | 1. |
| Pfam | PF02151 | UVR | 1. |
| SMART | SM00487 | DEXDc | 1. |
| SMART | SM00490 | HELICc | 1. |
| TIGRFAMs | TIGR00631 | uvrb | 1. |
| PROSITE | PS50151 | UVR | 1. |
Keywords:
ATP-binding; Complete proteome; DNA damage; DNA excision; DNA repair; Excision nuclease; Nucleotide-binding; SOS response.
References:
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=O157:H7 / EDL933 / ATCC 700927 / EHEC;
RX MEDLINE=21074935; PubMed=11206551; DOI=10.1038/35054089;
RA Perna N.T., Plunkett G. III, Burland V., Mau B., Glasner J.D.,
RA Rose D.J., Mayhew G.F., Evans P.S., Gregor J., Kirkpatrick H.A.,
RA Posfai G., Hackett J., Klink S., Boutin A., Shao Y., Miller L.,
RA Grotbeck E.J., Davis N.W., Lim A., Dimalanta E.T., Potamousis K.,
RA Apodaca J., Anantharaman T.S., Lin J., Yen G., Schwartz D.C.,
RA Welch R.A., Blattner F.R.;
RT "Genome sequence of enterohaemorrhagic Escherichia coli O157:H7.";
RL Nature 409:529-533(2001).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=O157:H7 / Sakai / RIMD 0509952 / EHEC;
RX MEDLINE=21156231; PubMed=11258796; DOI=10.1093/dnares/8.1.11;
RA Hayashi T., Makino K., Ohnishi M., Kurokawa K., Ishii K., Yokoyama K.,
RA Han C.-G., Ohtsubo E., Nakayama K., Murata T., Tanaka M., Tobe T.,
RA Iida T., Takami H., Honda T., Sasakawa C., Ogasawara N., Yasunaga T.,
RA Kuhara S., Shiba T., Hattori M., Shinagawa H.;
RT "Complete genome sequence of enterohemorrhagic Escherichia coli
RT O157:H7 and genomic comparison with a laboratory strain K-12.";
RL DNA Res. 8:11-22(2001).
Feature:
INIT_MET 0 0 By similarity.
CHAIN 1 672 UvrABC system protein B.
/FTId=PRO_0000138391.
DOMAIN 632 667 UVR.
NP_BIND 38 45 ATP (Potential).
MOTIF 91 114 Beta-hairpin.
SITE 629 630 Cleavage (Potential).
Comments:
-!- FUNCTION: The UvrABC repair system catalyzes the recognition and
processing of DNA lesions. A damage recognition complex composed
of 2 uvrA and 2 uvrB subunits scans DNA for abnormalities. Upon
binding of the uvrA(2)B(2) complex to a putative damaged site, the
DNA wraps around one uvrB monomer. DNA wrap is dependent on ATP
binding by uvrB and probably causes local melting of the DNA
helix, facilitating insertion of uvrB beta-hairpin between the DNA
strands. Then uvrB probes one DNA strand for the presence of a
lesion. If a lesion is found the uvrA subunits dissociate and the
uvrB-DNA preincision complex is formed. This complex is
subsequently bound by uvrC and the second uvrB is released. If no
lesion is found, the DNA wraps around the other uvrB subunit that
will check the other stand for damage (By similarity).
-!- SUBUNIT: Forms a heterotetramer with uvrA during the search for
lesions. Interacts with uvrC in an incision complex (By
similarity).
-!- SUBCELLULAR LOCATION: Cytoplasm (By similarity).
-!- DOMAIN: The beta-hairpin motif is involved in DNA binding (By
similarity).
-!- SIMILARITY: Belongs to the uvrB family.
-!- SIMILARITY: Contains 1 UVR domain.
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Sequence length: 672
SKPFKLNSAF KPSGDQPEAI RRLEEGLEDG LAHQTLLGVT GSGKTFTIAN VIADLQRPTM
VLAPNKTLAA QLYGEMKEFF PENAVEYFVS YYDYYQPEAY VPSSDTFIEK DASVNEHIEQ
MRLSATKAML ERRDVVVVAS VSAIYGLGDP DLYLKMMLHL TVGMIIDQRA ILRRLAELQY
ARNDQAFQRG TFRVRGEVID IFPAESDDIA LRVELFDEEV ERLSLFDPLT GQIVSTIPRF
TIYPKTHYVT PRERIVQAME EIKEELAARR KVLLENNKLL EEQRLTQRTQ FDLEMMNELG
YCSGIENYSR FLSGRGPGEP PPTLFDYLPA DGLLVVDESH VTIPQIGGMY RGDRARKETL
VEYGFRLPSA LDNRPLKFEE FEALAPQTIY VSATPGNYEL EKSGGDVVDQ VVRPTGLLDP
IIEVRPVATQ VDDLLSEIRQ RAAINERVLV TTLTKRMAED LTEYLEEHGE RVRYLHSDID
TVERMEIIRD LRLGEFDVLV GINLLREGLD MPEVSLVAIL DADKEGFLRS ERSLIQTIGR
AARNVNGKAI LYGDKITPSM AKAIGETERR REKQQKYNEE HGITPQGLNK KVVDILALGQ
NIAKTKAKGR GKSRPIVEPD NVPMDMSPKA LQQKIHELEG LMMQHAQNLE FEEAAQIRDQ
LHQLRELFIA AS