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Description:
DNA ligase (EC 6.5.1.2) (Polydeoxyribonucleotide synthase [NAD+]).
Molecular weight: 74156
View which proteins in this organism that is involved with DNA Repair;
classified after biological processes (using data from the GOA project):
DNA repair( GO:0006281 )
Important dates:
30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
01-MAY-1999, sequence version 1.
07-MAR-2006, entry version 39.
Phylogenetic order:
Bacteria Proteobacteria Epsilonproteobacteria Campylobacterales Helicobacteraceae Helicobacter.
To calculate the pI (Isoelectric point - the pH where a protein has a neutral charge),
go to this page and enter the protein ID (e.g 3MG_ECOLI): http://us.expasy.org/tools/pi_tool.html
Links to references in other databases for protein DNLJ_HELPJ:
| Database | Pointer | Add. info#1 | Add. info#2 |
| EMBL | AE001489 | AAD06141.1 | - |
| PIR | A71916 | A71916. | |
| HSSP | O87703 | 1B04 | |
| GenomeReviews | AE001439_GR | JHP0558.1 | |
| BioCyc | HPYL85963:JHP0558-MONOMER | -.1 | |
| InterPro | IPR001357 | BRCT. | |
| InterPro | IPR004150 | DNA_ligase_OB. | |
| InterPro | IPR001679 | DNAligase. | |
| InterPro | IPR003583 | HHH1_bd. | |
| InterPro | IPR004149 | Znf_DNAligase_C4. | |
| PANTHER | PTHR11107 | DNAligase.1 | 1. |
| Pfam | PF00533 | BRCT | 1. |
| Pfam | PF01653 | DNA_ligase_aden | 1. |
| Pfam | PF03120 | DNA_ligase_OB | 1. |
| Pfam | PF03119 | DNA_ligase_ZBD | 1. |
| ProDom | PD003944 | DNAligase | 1. |
| SMART | SM00292 | BRCT | 1. |
| SMART | SM00278 | HhH1 | 2. |
| SMART | SM00532 | LIGANc | 1. |
| TIGRFAMs | TIGR00575 | dnlj | 1. |
| PROSITE | PS50172 | BRCT | 1. |
| PROSITE | PS01055 | DNA_LIGASE_N1 | 1. |
| PROSITE | PS01056 | DNA_LIGASE_N2 | 1. |
Keywords:
Complete proteome; DNA damage; DNA repair; DNA replication; Ligase; NAD.
References:
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX MEDLINE=99120557; PubMed=9923682; DOI=10.1038/16495;
RA Alm R.A., Ling L.-S.L., Moir D.T., King B.L., Brown E.D., Doig P.C.,
RA Smith D.R., Noonan B., Guild B.C., deJonge B.L., Carmel G.,
RA Tummino P.J., Caruso A., Uria-Nickelsen M., Mills D.M., Ives C.,
RA Gibson R., Merberg D., Mills S.D., Jiang Q., Taylor D.E., Vovis G.F.,
RA Trust T.J.;
RT "Genomic sequence comparison of two unrelated isolates of the human
RT gastric pathogen Helicobacter pylori.";
RL Nature 397:176-180(1999).
Feature:
CHAIN 1 656 DNA ligase.
/FTId=PRO_0000161748.
DOMAIN 577 656 BRCT.
ACT_SITE 112 112 N6-AMP-lysine intermediate (By
similarity).
Comments:
-!- FUNCTION: This protein catalyzes the formation of phosphodiester
linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-
stranded DNA using NAD as a coenzyme and as the energy source for
the reaction. It is essential for DNA replication and repair of
damaged DNA.
-!- CATALYTIC ACTIVITY: NAD(+) + (deoxyribonucleotide)(n) +
(deoxyribonucleotide)(m) = AMP + nicotinamide nucleotide +
(deoxyribonucleotide)(n+m).
-!- SIMILARITY: Belongs to the NAD-dependent DNA ligase family.
-!- SIMILARITY: Contains 1 BRCT domain.
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Sequence length: 656
MIKSQKEYLE RIEYLNTLSH HYYNLDEPIV SDAVYDELYQ ELKAYEEENP NRIQANSPTQ
KVGATATNEF SKNPHLMRMW SLDDVFNQNE LRAWLQRILK VYPNASFVCS PKLDGVSLNL
LYQHGKLISA TTRGNGLEGE LVTNNAKHIA NIPHSIAYNG EIEIRGEVII SKEDFDALNK
ERLNANEPLF ANPRNAASGS LRQLDSNITK KRKLQFIPWG VGKHSLHFLR FKECLDFIVS
LGFSAIQYLS LNKNHQEIEE NYHTLIRERE GFFALLDGMV IVVDELDIQK ELGYTQKSPK
FACAYKFPAL EKHTKIVGVI NQVGRSGAIT PVALLEPVEI AGAMVNRATL HNYSEIEKKN
IMLNDRVVVI RSGDVIPKII KPLESYRDGS QQKIMRPKVC PICSHELLCE EIFTYCQNLN
CPARLKESLI HFASKDALNI QGLGDKVIEQ LFEEKLIFNA LDLYALKLED LMRLDKFKIK
KAQNLLDAIQ KSKNPPLWRL INALGIEHIG KGASKTLAKY GLNVLEKSEA EFLEMEGFGV
EMAHSLVNFY ASNQEFIRSL FELLNPKSSD TAEEKQKSSS VFSDKTIVLT GTLSKPRQEY
AQMLENLGAK IASSVSAKTD FLIAGENAGS KLALAQKHGV SVLNEEELLK RLKELD