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Description:
UvrABC system protein B (Protein uvrB) (Excinuclease ABC subunit B).
Molecular weight: 75927
View which proteins in this organism that is involved with DNA Repair;
classified after biological processes (using data from the GOA project):
DNA repair( GO:0006281 ) base-excision repair( GO:0006284 ) nucleotide-excision repair (and GO:0045001, a synonym)( GO:0006289 )
Important dates:
30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
01-MAY-1999, sequence version 1.
07-MAR-2006, entry version 36.
Phylogenetic order:
Bacteria Proteobacteria Epsilonproteobacteria Campylobacterales Helicobacteraceae Helicobacter.
To calculate the pI (Isoelectric point - the pH where a protein has a neutral charge),
go to this page and enter the protein ID (e.g 3MG_ECOLI): http://us.expasy.org/tools/pi_tool.html
Links to references in other databases for protein UVRB_HELPJ:
| Database | Pointer | Add. info#1 | Add. info#2 |
| EMBL | AE001532 | AAD06620.1 | - |
| PIR | C71856 | C71856. | |
| HSSP | P56981 | 1D9X | |
| GenomeReviews | AE001439_GR | JHP1041.1 | |
| BioCyc | HPYL85963:JHP1041-MONOMER | -.1 | |
| HAMAP | MF_00204 | - | 1. |
| InterPro | IPR001410 | DEAD. | |
| InterPro | IPR011545 | DEAD/DEAH_N. | |
| InterPro | IPR001650 | Helicase_C. | |
| InterPro | IPR006935 | ResIII. | |
| InterPro | IPR001943 | UvrB/C. | |
| InterPro | IPR004807 | UvrB_ABC. | |
| Pfam | PF00271 | Helicase_C | 1. |
| Pfam | PF04851 | ResIII | 1. |
| Pfam | PF02151 | UVR | 1. |
| SMART | SM00487 | DEXDc | 1. |
| SMART | SM00490 | HELICc | 1. |
| TIGRFAMs | TIGR00631 | uvrb | 1. |
| PROSITE | PS50151 | UVR | 1. |
Keywords:
ATP-binding; Complete proteome; DNA damage; DNA excision; DNA repair; Excision nuclease; Nucleotide-binding; SOS response.
References:
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX MEDLINE=99120557; PubMed=9923682; DOI=10.1038/16495;
RA Alm R.A., Ling L.-S.L., Moir D.T., King B.L., Brown E.D., Doig P.C.,
RA Smith D.R., Noonan B., Guild B.C., deJonge B.L., Carmel G.,
RA Tummino P.J., Caruso A., Uria-Nickelsen M., Mills D.M., Ives C.,
RA Gibson R., Merberg D., Mills S.D., Jiang Q., Taylor D.E., Vovis G.F.,
RA Trust T.J.;
RT "Genomic sequence comparison of two unrelated isolates of the human
RT gastric pathogen Helicobacter pylori.";
RL Nature 397:176-180(1999).
Feature:
CHAIN 1 658 UvrABC system protein B.
/FTId=PRO_0000138397.
DOMAIN 623 658 UVR.
NP_BIND 38 45 ATP (Potential).
MOTIF 91 114 Beta-hairpin.
Comments:
-!- FUNCTION: The UvrABC repair system catalyzes the recognition and
processing of DNA lesions. A damage recognition complex composed
of 2 uvrA and 2 uvrB subunits scans DNA for abnormalities. Upon
binding of the uvrA(2)B(2) complex to a putative damaged site, the
DNA wraps around one uvrB monomer. DNA wrap is dependent on ATP
binding by uvrB and probably causes local melting of the DNA
helix, facilitating insertion of uvrB beta-hairpin between the DNA
strands. Then uvrB probes one DNA strand for the presence of a
lesion. If a lesion is found the uvrA subunits dissociate and the
uvrB-DNA preincision complex is formed. This complex is
subsequently bound by uvrC and the second uvrB is released. If no
lesion is found, the DNA wraps around the other uvrB subunit that
will check the other stand for damage (By similarity).
-!- SUBUNIT: Forms a heterotetramer with uvrA during the search for
lesions. Interacts with uvrC in an incision complex (By
similarity).
-!- SUBCELLULAR LOCATION: Cytoplasm (By similarity).
-!- DOMAIN: The beta-hairpin motif is involved in DNA binding (By
similarity).
-!- SIMILARITY: Belongs to the uvrB family.
-!- SIMILARITY: Contains 1 UVR domain.
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Sequence length: 658
MPLFDLKSPY PPAGDQPQAI EALTKSLKNN NHYQTLVGVT GSGKTYTMAN IIAQTNKPAL
IMSHNKTLCA QLYSEFKAFF PHNRVEYFIS HFDYYQPESY IPRRDLFIEK DSSINDDLER
LRLSATTSLL GYDDVIVIAS VSANYGLGNP EEYLKVMEKI KVGEKRAYKS FLLKLVEMGY
SRNEVVFDRG SFRATGECVD IFPAYNDAEF IRIEFFGDEI ERIAVFDALE RNEIKRLDSV
MLYAASQFAV GSERLNLAVK SIEDELALRL KFFKEQDKML EYNRLKQRTE YDLEMISATG
VCKGIENYAR HFTGKAPNET PFCLFDYLGI FEREFLVIVD ESHVSLPQFG GMYAGDMSRK
SVLVEYGFRL PSALDNRPLK FDEFIHKNCQ FLFVSATPNK LELELSQKNV AEQIIRPTGL
LDPKFEVRDS DKQVQDLFDE IKSVVARGER VLITTLTKKM AEELCKYYAE WGLKVRYMHS
EIDAIERNHI IRSLRLKEFD VLIGINLLRE GLDLPEVSLV AIMDADKEGF LRSETSLIQT
MGRAARNANG KVLLYAKKTT QSMQKAFEIT SYRRAKQEEF NKIHNITPKT VTRALEEELK
LRDDEIKIAK ALKKDKMPKS EREKIIKELD KKMRERAKNL DFEEAMRLRD EIAQLRTL