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Description:
DNA ligase (EC 6.5.1.2) (Polydeoxyribonucleotide synthase [NAD+]).
Molecular weight: 74242
View which proteins in this organism that is involved with DNA Repair;
classified after biological processes (using data from the GOA project):
DNA repair( GO:0006281 )
Important dates:
14-AUG-2001, integrated into UniProtKB/Swiss-Prot.
01-OCT-2000, sequence version 1.
07-MAR-2006, entry version 35.
Phylogenetic order:
Bacteria Firmicutes Bacillales Bacillaceae Bacillus.
To calculate the pI (Isoelectric point - the pH where a protein has a neutral charge),
go to this page and enter the protein ID (e.g 3MG_ECOLI): http://us.expasy.org/tools/pi_tool.html
Links to references in other databases for protein DNLJ_BACHD:
| Database | Pointer | Add. info#1 | Add. info#2 |
| EMBL | BA000004 | BAB04368.1 | - |
| PIR | A83731 | A83731. | |
| HSSP | O87703 | 1B04 | |
| GenomeReviews | BA000004_GR | BH0649.1 | |
| BioCyc | BHAL86665:BH0649-MONOMER | -.1 | |
| InterPro | IPR001357 | BRCT. | |
| InterPro | IPR004150 | DNA_ligase_OB. | |
| InterPro | IPR001679 | DNAligase. | |
| InterPro | IPR003583 | HHH1_bd. | |
| InterPro | IPR004149 | Znf_DNAligase_C4. | |
| PANTHER | PTHR11107 | DNAligase.1 | 1. |
| Pfam | PF00533 | BRCT | 1. |
| Pfam | PF01653 | DNA_ligase_aden | 1. |
| Pfam | PF03120 | DNA_ligase_OB | 1. |
| Pfam | PF03119 | DNA_ligase_ZBD | 1. |
| ProDom | PD003944 | DNAligase | 1. |
| SMART | SM00292 | BRCT | 1. |
| SMART | SM00278 | HhH1 | 3. |
| SMART | SM00532 | LIGANc | 1. |
| TIGRFAMs | TIGR00575 | dnlj | 1. |
| PROSITE | PS50172 | BRCT | 1. |
| PROSITE | PS01055 | DNA_LIGASE_N1 | 1. |
| PROSITE | PS01056 | DNA_LIGASE_N2 | 1. |
Keywords:
Complete proteome; DNA damage; DNA repair; DNA replication; Ligase; NAD.
References:
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C-125 / JCM 9153;
RX MEDLINE=20512582; PubMed=11058132; DOI=10.1093/nar/28.21.4317;
RA Takami H., Nakasone K., Takaki Y., Maeno G., Sasaki R., Masui N.,
RA Fuji F., Hirama C., Nakamura Y., Ogasawara N., Kuhara S.,
RA Horikoshi K.;
RT "Complete genome sequence of the alkaliphilic bacterium Bacillus
RT halodurans and genomic sequence comparison with Bacillus subtilis.";
RL Nucleic Acids Res. 28:4317-4331(2000).
Feature:
CHAIN 1 669 DNA ligase.
/FTId=PRO_0000161736.
DOMAIN 591 669 BRCT.
ACT_SITE 115 115 N6-AMP-lysine intermediate (By
similarity).
Comments:
-!- FUNCTION: This protein catalyzes the formation of phosphodiester
linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-
stranded DNA using NAD as a coenzyme and as the energy source for
the reaction. It is essential for DNA replication and repair of
damaged DNA (By similarity).
-!- CATALYTIC ACTIVITY: NAD(+) + (deoxyribonucleotide)(n) +
(deoxyribonucleotide)(m) = AMP + nicotinamide nucleotide +
(deoxyribonucleotide)(n+m).
-!- SIMILARITY: Belongs to the NAD-dependent DNA ligase family.
-!- SIMILARITY: Contains 1 BRCT domain.
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Sequence length: 669
MERNDAERKI EKLRNQLEEY GYHYYVLDKP LVSDAEYDGL MNELIELEEA FPELKSDTSP
SVRVGGPPLP HFEKVEHRTP MLSLGNAFSD QDLRDFDRRV RQVVGDEVTY SCELKIDGLA
ISLIYESGRF VRGATRGDGT TGEDITQNLR TIPSIPLRLK EAVSLEVRGE AFMPKRSFEA
LNEAKEAAGE ERFANPRNAA AGSLRQLDPK LAAKRHLDAF IYAIGSVEGK ELHSHHEGLN
YLKTIGFKIN PESAYCESID EVIDYVNSWL ERRADLPYEI DGIVIKVDNV NFQEQLGYTA
KSPRWAIAYK FPAEEVITTL LDITLNVGRT GVVTPTAELK PVTVAGTTVK RASLHNEDLI
REKDIRLGDS VVVKKAGDII PEVVNVLTEL RTGEERPFSM PTHCPECGSE LVRLEGEVAL
RCINPQCPAQ IREGLIHFVS RQAMNIDGLG EKVITQLFEH GLIHNVADLY KLNKDELLQL
ERMGEKSVNN LLASIETSKK SSLERLLFGL GIRFVGSKAA KTLAMHFPTM TELRRASYDE
LIAVNEIGEK MAASIVSYFE KPEVNELIDE LASLGVNMTY NGPKPIGEEE IADSPFAGKT
VVLTGKLQAL TRGEAKEKIE ALGGKVTGSV SKNTDLLVAG EDAGSKLTKA KELNIEIWDE
EALVKAISH