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Description:
LexA repressor (EC 3.4.21.88).
Molecular weight: 23134
View which proteins in this organism that is involved with DNA Repair;
classified after biological processes (using data from the GOA project):
DNA repair( GO:0006281 )
Important dates:
27-APR-2001, integrated into UniProtKB/Swiss-Prot.
01-OCT-2000, sequence version 1.
07-MAR-2006, entry version 38.
Phylogenetic order:
Bacteria Firmicutes Bacillales Bacillaceae Bacillus.
To calculate the pI (Isoelectric point - the pH where a protein has a neutral charge),
go to this page and enter the protein ID (e.g 3MG_ECOLI): http://us.expasy.org/tools/pi_tool.html
Links to references in other databases for protein LEXA_BACHD:
| Database | Pointer | Add. info#1 | Add. info#2 |
| EMBL | BA000004 | BAB06075.1 | - |
| PIR | D83944 | D83944. | |
| HSSP | P03033 | 1JHC | |
| GenomeReviews | BA000004_GR | BH2356.1 | |
| BioCyc | BHAL86665:BH2356-MONOMER | -.1 | |
| HAMAP | MF_00015 | - | 1. |
| InterPro | IPR006199 | LexA_DNA_bd. | |
| InterPro | IPR006200 | Pept_S24_LexA. | |
| InterPro | IPR006198 | Pept_S24_S26. | |
| InterPro | IPR006197 | Pept_S24_SOS. | |
| InterPro | IPR011991 | Wing_hlx_DNA_bd. | |
| Pfam | PF01726 | LexA_DNA_bind | 1. |
| Pfam | PF00717 | Peptidase_S24 | 1. |
| PRINTS | PR00726 | LEXASERPTASE. | |
| TIGRFAMs | TIGR00498 | lexA | 1. |
Keywords:
Autocatalytic cleavage; Complete proteome; DNA damage; DNA repair; DNA replication; DNA-binding; Hydrolase; Repressor; SOS response; Transcription; Transcription regulation.
References:
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C-125 / JCM 9153;
RX MEDLINE=20512582; PubMed=11058132; DOI=10.1093/nar/28.21.4317;
RA Takami H., Nakasone K., Takaki Y., Maeno G., Sasaki R., Masui N.,
RA Fuji F., Hirama C., Nakamura Y., Ogasawara N., Kuhara S.,
RA Horikoshi K.;
RT "Complete genome sequence of the alkaliphilic bacterium Bacillus
RT halodurans and genomic sequence comparison with Bacillus subtilis.";
RL Nucleic Acids Res. 28:4317-4331(2000).
Feature:
CHAIN 1 207 LexA repressor.
/FTId=PRO_0000170006.
DNA_BIND 28 48 H-T-H motif (By similarity).
ACT_SITE 129 129 Involved in auto-cleavage (By
similarity).
ACT_SITE 167 167 Involved in auto-cleavage (By
similarity).
SITE 94 95 Cleavage (auto-) (By similarity).
Comments:
-!- FUNCTION: Represses a number of genes involved in the response to
DNA damage (SOS response), including recA and lexA. In the
presence of single-stranded DNA, recA interacts with lexA causing
an autocatalytic cleavage which disrupts the DNA-binding part of
lexA, leading to derepression of the SOS regulon and eventually
DNA repair (By similarity).
-!- CATALYTIC ACTIVITY: Hydrolysis of Ala-|-Gly bond in repressor
lexA.
-!- SUBUNIT: Homodimer (By similarity).
-!- SIMILARITY: Belongs to the peptidase S24 family.
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Sequence length: 207
MSKLSRRQQE ILDYIKEEVR AKGYPPSVRE IGEAVGLASS STVHGHLSRL EKKGYIRRDP
TKPRAIEVLD LENLASETTE AKATYIPVVG KVTAGLPITA VENVEEYFPL PEQLTANDNT
YALRIQGDSM IEAGIFDGDL VIVRQQQTAD NGDIIVAMTE EDEATVKRFF REKDYIRLQP
ENSTMEPIIL TTCTILGKVI GVFRTIH