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Description:
UvrABC system protein B (Protein uvrB) (Excinuclease ABC subunit B).
Molecular weight: 75590
View which proteins in this organism that is involved with DNA Repair;
classified after biological processes (using data from the GOA project):
DNA repair( GO:0006281 ) base-excision repair( GO:0006284 ) nucleotide-excision repair (and GO:0045001, a synonym)( GO:0006289 )
Important dates:
01-JUN-2001, integrated into UniProtKB/Swiss-Prot.
01-OCT-2000, sequence version 1.
07-MAR-2006, entry version 36.
Phylogenetic order:
Bacteria Firmicutes Bacillales Bacillaceae Bacillus.
To calculate the pI (Isoelectric point - the pH where a protein has a neutral charge),
go to this page and enter the protein ID (e.g 3MG_ECOLI): http://us.expasy.org/tools/pi_tool.html
Links to references in other databases for protein UVRB_BACHD:
| Database | Pointer | Add. info#1 | Add. info#2 |
| EMBL | BA000004 | BAB07314.1 | - |
| PIR | C84099 | C84099. | |
| HSSP | P56981 | 1D9X | |
| SMR | Q9K6X9 | 5-595.1 | |
| GenomeReviews | BA000004_GR | BH3595.1 | |
| BioCyc | BHAL86665:BH3595-MONOMER | -.1 | |
| HAMAP | MF_00204 | - | 1. |
| InterPro | IPR001410 | DEAD. | |
| InterPro | IPR011545 | DEAD/DEAH_N. | |
| InterPro | IPR001650 | Helicase_C. | |
| InterPro | IPR006935 | ResIII. | |
| InterPro | IPR001943 | UvrB/C. | |
| InterPro | IPR004807 | UvrB_ABC. | |
| Pfam | PF00271 | Helicase_C | 1. |
| Pfam | PF04851 | ResIII | 1. |
| Pfam | PF02151 | UVR | 1. |
| SMART | SM00487 | DEXDc | 1. |
| SMART | SM00490 | HELICc | 1. |
| TIGRFAMs | TIGR00631 | uvrb | 1. |
| PROSITE | PS50151 | UVR | 1. |
Keywords:
ATP-binding; Complete proteome; DNA damage; DNA excision; DNA repair; Excision nuclease; Nucleotide-binding; SOS response.
References:
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C-125 / JCM 9153;
RX MEDLINE=20512582; PubMed=11058132; DOI=10.1093/nar/28.21.4317;
RA Takami H., Nakasone K., Takaki Y., Maeno G., Sasaki R., Masui N.,
RA Fuji F., Hirama C., Nakamura Y., Ogasawara N., Kuhara S.,
RA Horikoshi K.;
RT "Complete genome sequence of the alkaliphilic bacterium Bacillus
RT halodurans and genomic sequence comparison with Bacillus subtilis.";
RL Nucleic Acids Res. 28:4317-4331(2000).
Feature:
CHAIN 1 660 UvrABC system protein B.
/FTId=PRO_0000138380.
DOMAIN 624 659 UVR.
NP_BIND 39 46 ATP (Potential).
MOTIF 92 115 Beta-hairpin.
Comments:
-!- FUNCTION: The UvrABC repair system catalyzes the recognition and
processing of DNA lesions. A damage recognition complex composed
of 2 uvrA and 2 uvrB subunits scans DNA for abnormalities. Upon
binding of the uvrA(2)B(2) complex to a putative damaged site, the
DNA wraps around one uvrB monomer. DNA wrap is dependent on ATP
binding by uvrB and probably causes local melting of the DNA
helix, facilitating insertion of uvrB beta-hairpin between the DNA
strands. Then uvrB probes one DNA strand for the presence of a
lesion. If a lesion is found the uvrA subunits dissociate and the
uvrB-DNA preincision complex is formed. This complex is
subsequently bound by uvrC and the second uvrB is released. If no
lesion is found, the DNA wraps around the other uvrB subunit that
will check the other stand for damage (By similarity).
-!- SUBUNIT: Forms a heterotetramer with uvrA during the search for
lesions. Interacts with uvrC in an incision complex (By
similarity).
-!- SUBCELLULAR LOCATION: Cytoplasm (By similarity).
-!- DOMAIN: The beta-hairpin motif is involved in DNA binding (By
similarity).
-!- SIMILARITY: Belongs to the uvrB family.
-!- SIMILARITY: Contains 1 UVR domain.
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Sequence length: 660
MSQSFELVSQ YKPQGDQPNA IRQLVAGINE GKKHQTLLGA TGTGKTFTMS NVIQQVNKPT
LVIAHNKTLA GQLYSEFKEF FPNNAVEYFV SYYDYYQPEA YVPQSDTYIE KDASINDEID
KLRHSATSAL FERNDVIIVA SVSCIYGLGS PEEYKELVCS LRTGMEIERN DLLRQLVDIQ
YDRNDVNFTR GTFRVRGDVV EIFPASRDEQ CIRVEFFGDE IDRITEVDAL TGEIKGERNH
VAIFPASHFV TREEKLKRAT KSIEAELEER LKELHDRGKL LEAQRLEQRT RYDLEMIHEM
GFCSGIENYS RHLTLRKAGE TPYTLLDFFP DDFLIIIDES HVTIPQIRAM YNGDQARKGV
LVDHGFRLPS ALDNRPLKFE EFEHKVHQAV FVSATPGPYE LEHTPEMVEQ IIRPTGLLDP
VIEVRPIEGQ IDDLIGEINE RVAKNERVLV TTLTKKMAED LTDYLKEVGI KVRYLHSEVK
TLERIEIIRQ LRLGTFNVLV GINLLREGLD IPEVSLVAIL DADKEGFLRA ERSLIQTIGR
AARNANGYVI MYADRMTKSM QIAIDETKRR RSIQEEYNRK HGITPKTIEK RIPDVIKATA
MVAEDGEEYT SHAPKQKMSK KEREAVIERM EAEMKEAAKT LNFERAAELR DLILELKAEG