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Description:
CDK-activating kinase assembly factor MAT1 (RING finger protein MAT1)(Menage a trois) (CDK7/cyclin H assembly factor) (p36) (p35) (CyclinG1-interacting protein) (RING finger protein 66).
Molecular weight: 35823
View which proteins in this organism that is involved with DNA Repair;
classified after biological processes (using data from the GOA project):
DNA repair( GO:0006281 )
Important dates:
01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
01-OCT-1996, sequence version 1.
07-FEB-2006, entry version 61.
Phylogenetic order:
Eukaryota Metazoa Chordata Craniata Vertebrata Euteleostomi Mammalia Eutheria Euarchontoglires Primates Catarrhini Hominidae Homo.
To calculate the pI (Isoelectric point - the pH where a protein has a neutral charge),
go to this page and enter the protein ID (e.g 3MG_ECOLI): http://us.expasy.org/tools/pi_tool.html
Links to references in other databases for protein MAT1_HUMAN:
| Database | Pointer | Add. info#1 | Add. info#2 |
| EMBL | X87843 | CAA61112.1 | - |
| EMBL | X92669 | CAA63356.1 | - |
| EMBL | U61835 | AAB05248.1 | - |
| EMBL | AY165512 | AAN47195.1 | - |
| EMBL | BC000820 | AAH00820.1 | - |
| PIR | S60157 | S60157. | |
| PDB | 1G25 | NMR | A=1-65. |
| TRANSFAC | T02187 | -. | |
| Ensembl | ENSG00000020426 | Homo sapiens.1 | |
| H-InvDB | HIX0011713 | -.1 | |
| HGNC | HGNC:7181 | MNAT1.1 | |
| MIM | 602659 | gene. | |
| Reactome | P51948 | -.1 | |
| GO | GO:0008270 | F:zinc ion binding | TAS. |
| GO | GO:0008283 | P:cell proliferation | TAS. |
| GO | GO:0006281 | P:DNA repair | TAS. |
| GO | GO:0006461 | P:protein complex assembly | TAS. |
| GO | GO:0000079 | P:regulation of cyclin dependent protein kina... | TAS. |
| GO | GO:0006357 | P:regulation of transcription from RNA polyme... | TAS. |
| InterPro | IPR004575 | MAT1. | |
| InterPro | IPR003903 | UIM. | |
| InterPro | IPR001841 | Znf_RING. | |
| PANTHER | PTHR12683 | MAT.1 | 1. |
| Pfam | PF06391 | MAT1 | 1. |
| SMART | SM00184 | RING | 1. |
| TIGRFAMs | TIGR00570 | cdk7 | 1. |
| PROSITE | PS50330 | UIM | 1. |
| PROSITE | PS00518 | ZF_RING_1 | 1. |
| PROSITE | PS50089 | ZF_RING_2 | 1. |
Keywords:
3D-structure; Cell cycle; Direct protein sequencing; Metal-binding; Nuclear protein; Transcription; Transcription regulation; Zinc; Zinc-finger.
References:
RN [1]
RP NUCLEOTIDE SEQUENCE, AND PROTEIN SEQUENCE OF 23-48 AND 123-147.
RC TISSUE=Placenta;
RX MEDLINE=96091133; PubMed=8521818;
RA Tassan J.-P., Jaquenoud M., Fry A.M., Frutiger S., Hughes G.J.,
RA Nigg E.A.;
RT "In vitro assembly of a functional human CDK7-cyclin H complex
RT requires MAT1, a novel 36 kDa RING finger protein.";
RL EMBO J. 14:5608-5617(1995).
RN [2]
RP NUCLEOTIDE SEQUENCE.
RC TISSUE=Cervix carcinoma;
RX MEDLINE=96105021; PubMed=8521393;
RA Yee A., Nichols M., Wu L., Hall F.L., Kobayashi R., Xiong Y.;
RT "Molecular cloning of CDK7-associated human MAT1, a cyclin-dependent
RT kinase-activating kinase (CAK) assembly factor.";
RL Cancer Res. 55:6058-6062(1995).
RN [3]
RP NUCLEOTIDE SEQUENCE.
RA Xu F., Hall F.L., Starnes V., Wu L.;
RL Submitted (JUN-1996) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RA Rieder M.J., Livingston R.J., Daniels M.R., Montoya M.A., Chung M.-W.,
RA Miyamoto K.E., Nguyen C.P., Nguyen D.A., Poel C.L., Robertson P.D.,
RA Schackwitz W.S., Sherwood J.K., Witrak L.A., Nickerson D.A.;
RT "NIEHS-SNPs, environmental genome project, NIEHS ES15478, Department
RT of Genome Sciences, Seattle, WA (URL: http://egp.gs.washington.edu).";
RL Submitted (OCT-2002) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Placenta;
RX MEDLINE=22388257; PubMed=12477932; DOI=10.1073/pnas.242603899;
RA Strausberg R.L., Feingold E.A., Grouse L.H., Derge J.G.,
RA Klausner R.D., Collins F.S., Wagner L., Shenmen C.M., Schuler G.D.,
RA Altschul S.F., Zeeberg B., Buetow K.H., Schaefer C.F., Bhat N.K.,
RA Hopkins R.F., Jordan H., Moore T., Max S.I., Wang J., Hsieh F.,
RA Diatchenko L., Marusina K., Farmer A.A., Rubin G.M., Hong L.,
RA Stapleton M., Soares M.B., Bonaldo M.F., Casavant T.L., Scheetz T.E.,
RA Brownstein M.J., Usdin T.B., Toshiyuki S., Carninci P., Prange C.,
RA Raha S.S., Loquellano N.A., Peters G.J., Abramson R.D., Mullahy S.J.,
RA Bosak S.A., McEwan P.J., McKernan K.J., Malek J.A., Gunaratne P.H.,
RA Richards S., Worley K.C., Hale S., Garcia A.M., Gay L.J., Hulyk S.W.,
RA Villalon D.K., Muzny D.M., Sodergren E.J., Lu X., Gibbs R.A.,
RA Fahey J., Helton E., Ketteman M., Madan A., Rodrigues S., Sanchez A.,
RA Whiting M., Madan A., Young A.C., Shevchenko Y., Bouffard G.G.,
RA Blakesley R.W., Touchman J.W., Green E.D., Dickson M.C.,
RA Rodriguez A.C., Grimwood J., Schmutz J., Myers R.M.,
RA Butterfield Y.S.N., Krzywinski M.I., Skalska U., Smailus D.E.,
RA Schnerch A., Schein J.E., Jones S.J.M., Marra M.A.;
RT "Generation and initial analysis of more than 15,000 full-length human
RT and mouse cDNA sequences.";
RL Proc. Natl. Acad. Sci. U.S.A. 99:16899-16903(2002).
RN [6]
RP FUNCTION.
RX MEDLINE=99149021; PubMed=10024882; DOI=10.1016/S1097-2765(00)80177-X;
RA Tirode F., Busso D., Coin F., Egly J.-M.;
RT "Reconstitution of the transcription factor TFIIH: assignment of
RT functions for the three enzymatic subunits, XPB, XPD, and cdk7.";
RL Mol. Cell 3:87-95(1999).
RN [7]
RP STRUCTURE BY NMR OF 1-65.
RX PubMed=11056162; DOI=10.1074/jbc.M007963200;
RA Gervais V., Busso D., Wasielewski E., Poterszman A., Egly J.M.,
RA Thierry J.C., Kieffer B.;
RT "Solution structure of the N-terminal domain of the human TFIIH MAT1
RT subunit: new insights into the RING finger family.";
RL J. Biol. Chem. 276:7457-7464(2001).
Feature:
CHAIN 1 309 CDK-activating kinase assembly factor
MAT1.
/FTId=PRO_0000055932.
REPEAT 142 161 UIM.
ZN_FING 6 50 RING-type.
CONFLICT 188 229 Missing (in Ref. 3).
CONFLICT 247 248 EY -> DN (in Ref. 3).
TURN 3 4
STRAND 7 7
TURN 8 11
HELIX 12 15
STRAND 17 18
STRAND 21 23
TURN 25 26
STRAND 30 31
HELIX 32 40
TURN 41 42
STRAND 43 45
TURN 47 49
STRAND 54 55
STRAND 59 61
Comments:
-!- FUNCTION: Stabilizes the cyclin H-CDK7 complex to form a
functional CDK-activating kinase (CAK) enzymatic complex. CAK
activates the cyclin-associated kinases CDC2/CDK1, CDK2, CDK4 and
CDK6 by threonine phosphorylation. CAK complexed to the core-TFIIH
basal transcription factor activates RNA polymerase II by serine
phosphorylation of the repetitive carboxyl-terminus domain (CTD)
of its large subunit (POLR2A), allowing its escape from the
promoter and elongation of the transcripts. Involved in cell cycle
control and in RNA transcription by RNA polymerase II.
-!- SUBUNIT: Associates primarily with CDK7 and cyclin H to form the
CAK complex. CAK can further associate with the core-TFIIH to form
the TFIIH basal transcription factor.
-!- SUBCELLULAR LOCATION: Nucleus.
-!- TISSUE SPECIFICITY: Highest levels in colon and testis. Moderate
levels are present thymus, prostate, ovary, and small intestine.
The lowest levels are found in spleen and leukocytes.
-!- SIMILARITY: Contains 1 RING-type zinc finger.
-!- SIMILARITY: Contains 1 UIM (ubiquitin-interacting motif) repeat.
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Sequence length: 309
MDDQGCPRCK TTKYRNPSLK LMVNVCGHTL CESCVDLLFV RGAGNCPECG TPLRKSNFRV
QLFEDPTVDK EVEIRKKVLK IYNKREEDFP SLREYNDFLE EVEEIVFNLT NNVDLDNTKK
KMEIYQKENK DVIQKNKLKL TREQEELEEA LEVERQENEQ RRLFIQKEEQ LQQILKRKNK
QAFLDELESS DLPVALLLAQ HKDRSTQLEM QLEKPKPVKP VTFSTGIKMG QHISLAPIHK
LEEALYEYQP LQIETYGPHV PELEMLGRLG YLNHVRAASP QDLAGGYTSS LACHRALQDA
FSGLFWQPS