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Description:
DNA polymerase sigma (EC 2.7.7.7) (Topoisomerase-related functionprotein 4-1) (TRF4-1) (LAK-1) (DNA polymerase kappa).
Molecular weight: 59874
View which proteins in this organism that is involved with DNA Repair;
classified after biological processes (using data from the GOA project):
double-strand break repair( GO:0006302 )
Important dates:
01-FEB-2005, integrated into UniProtKB/Swiss-Prot.
01-FEB-2005, sequence version 2.
07-FEB-2006, entry version 13.
Phylogenetic order:
Eukaryota Metazoa Chordata Craniata Vertebrata Euteleostomi Mammalia Eutheria Euarchontoglires Primates Catarrhini Hominidae Homo.
To calculate the pI (Isoelectric point - the pH where a protein has a neutral charge),
go to this page and enter the protein ID (e.g 3MG_ECOLI): http://us.expasy.org/tools/pi_tool.html
Links to references in other databases for protein POLS_HUMAN:
| Database | Pointer | Add. info#1 | Add. info#2 |
| EMBL | AF089896 | AAD45198.1 | ALT_SEQ |
| EMBL | AB005754 | BAA24434.2.1 | - |
| EMBL | AY623114 | AAT38110.1 | - |
| EMBL | BC084567 | AAH84567.1 | ALT_INIT |
| Ensembl | ENSG00000112941 | Homo sapiens.1 | |
| HGNC | HGNC:16705 | POLS.1 | |
| MIM | 605198 | gene. | |
| GO | GO:0005634 | C:nucleus | NAS. |
| GO | GO:0003677 | F:DNA binding | TAS. |
| GO | GO:0006281 | P:DNA repair | NAS. |
| GO | GO:0007062 | P:sister chromatid cohesion | NAS. |
| InterPro | IPR002934 | DNA_pol_beta. | |
| InterPro | IPR001201 | PAP_25A_core. | |
| InterPro | IPR002058 | PAP_assoc. | |
| Pfam | PF01909 | NTP_transf_2 | 1. |
| Pfam | PF03828 | PAP_assoc | 1. |
Keywords:
Cell cycle; Cell division; DNA synthesis; DNA-binding; DNA-directed DNA polymerase; Mitosis; Nuclear protein; Nucleotidyltransferase; Polymorphism; Transferase.
References:
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX MEDLINE=99167497; PubMed=10066793; DOI=10.1074/jbc.274.11.7302;
RA Walowsky C., Fitzhugh D.J., Castano I.B., Ju J.Y., Levin N.A.,
RA Christman M.F.;
RT "The topoisomerase-related function gene TRF4 affects cellular
RT sensitivity to the antitumor agent camptothecin.";
RL J. Biol. Chem. 274:7302-7308(1999).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA].
RA Abe Y., Takaoka Y.;
RT "mLT positive LAK-cell clone No. 1.";
RL Submitted (JUL-1997) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND VARIANTS SER-326 AND SER-396.
RA Livingston R.J., Rieder M.J., Chung M.-W., Ritchie T.K., Olson A.N.,
RA Nguyen C.P., Nguyen D.A., Poel C.L., Robertson P.D., Schackwitz W.S.,
RA Sherwood J.K., Sherwood A.M., Leithauser B.J., Nickerson D.A.;
RT "NIEHS-SNPs, environmental genome project, NIEHS ES15478, Department
RT of Genome Sciences, Seattle, WA (URL: http://egp.gs.washington.edu).";
RL Submitted (MAY-2004) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Lung;
RX MEDLINE=22388257; PubMed=12477932; DOI=10.1073/pnas.242603899;
RA Strausberg R.L., Feingold E.A., Grouse L.H., Derge J.G.,
RA Klausner R.D., Collins F.S., Wagner L., Shenmen C.M., Schuler G.D.,
RA Altschul S.F., Zeeberg B., Buetow K.H., Schaefer C.F., Bhat N.K.,
RA Hopkins R.F., Jordan H., Moore T., Max S.I., Wang J., Hsieh F.,
RA Diatchenko L., Marusina K., Farmer A.A., Rubin G.M., Hong L.,
RA Stapleton M., Soares M.B., Bonaldo M.F., Casavant T.L., Scheetz T.E.,
RA Brownstein M.J., Usdin T.B., Toshiyuki S., Carninci P., Prange C.,
RA Raha S.S., Loquellano N.A., Peters G.J., Abramson R.D., Mullahy S.J.,
RA Bosak S.A., McEwan P.J., McKernan K.J., Malek J.A., Gunaratne P.H.,
RA Richards S., Worley K.C., Hale S., Garcia A.M., Gay L.J., Hulyk S.W.,
RA Villalon D.K., Muzny D.M., Sodergren E.J., Lu X., Gibbs R.A.,
RA Fahey J., Helton E., Ketteman M., Madan A., Rodrigues S., Sanchez A.,
RA Whiting M., Madan A., Young A.C., Shevchenko Y., Bouffard G.G.,
RA Blakesley R.W., Touchman J.W., Green E.D., Dickson M.C.,
RA Rodriguez A.C., Grimwood J., Schmutz J., Myers R.M.,
RA Butterfield Y.S.N., Krzywinski M.I., Skalska U., Smailus D.E.,
RA Schnerch A., Schein J.E., Jones S.J.M., Marra M.A.;
RT "Generation and initial analysis of more than 15,000 full-length human
RT and mouse cDNA sequences.";
RL Proc. Natl. Acad. Sci. U.S.A. 99:16899-16903(2002).
RN [5]
RP FUNCTION.
RX PubMed=10926539; DOI=10.1126/science.289.5480.774;
RA Wang Z., Castano I.B., De Las Penas A., Adams C., Christman M.F.;
RT "Pol kappa: a DNA polymerase required for sister chromatid cohesion.";
RL Science 289:774-779(2000).
RN [6]
RP REVIEW.
RX PubMed=11459963; DOI=10.1073/pnas.131022798;
RA Carson D.R., Christman M.F.;
RT "Evidence that replication fork components catalyze establishment of
RT cohesion between sister chromatids.";
RL Proc. Natl. Acad. Sci. U.S.A. 98:8270-8275(2001).
Feature:
CHAIN 1 542 DNA polymerase sigma.
/FTId=PRO_0000120308.
COMPBIAS 346 372 Ser-rich.
VARIANT 326 326 N -> S.
/FTId=VAR_021175.
VARIANT 396 396 G -> S.
/FTId=VAR_021176.
Comments:
-!- FUNCTION: DNA polymerase, probably involved in DNA repair. May
play a role in sister chromatid cohesion.
-!- CATALYTIC ACTIVITY: Deoxynucleoside triphosphate + DNA(n) =
diphosphate + DNA(n+1).
-!- SUBCELLULAR LOCATION: Nucleus (Probable).
-!- SIMILARITY: Belongs to the DNA polymerase type-B-like family.
-!- CAUTION: Ref.1 sequence differs from that shown due to a
frameshift in position 474.
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Sequence length: 542
MSPCPEEAAM RREVVKRIET VVKDLWPTAD VQIFGSFSTG LYLPTSDIDL VVFGKWERPP
LQLLEQALRK HNVAEPCSIK VLDKATVPII KLTDQETEVK VDISFNMETG VRAAEFIKNY
MKKYSLLPYL ILVLKQFLLQ RDLNEVFTGG ISSYSLILMA ISFLQLHPRI DARRADENLG
MLLVEFFELY GRNFNYLKTG IRIKEGGAYI AKEEIMKAMT SGYRPSMLCI EDPLLPGNDV
GRSSYGAMQV KQVFDYAYIV LSHAVSPLAR SYPNRDAEST LGRIIKVTQE VIDYRRWIKE
KWGSKAHPSP GMDSRIKIKE RIATCNGEQT QNREPESPYG QRLTLSLSSP QLLSSGSSAS
SVSSLSGSDV DSDTPPCTTP SVYQFSLQAP APLMAGLPTA LPMPSGKPQP TTSRTLIMTT
NNQTRFTIPP PTLGVAPVPC RQAGVEGTAS LKAVHHMSSP AIPSASPNPL SSPHLYHKQH
NGMKLSMKGS HGHTQGGGYS SVGSGGVRPP VGNRGHHQYN RTGWRRKKHT HTRDSLPVSL
SR