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Description:
Replication protein A 70 kDa DNA-binding subunit (RP-A) (RF-A)(Replication factor-A protein 1) (Single-stranded DNA-binding protein)(p70).
Molecular weight: 68138
View which proteins in this organism that is involved with DNA Repair;
classified after biological processes (using data from the GOA project):
DNA repair( GO:0006281 )
Important dates:
01-AUG-1992, integrated into UniProtKB/Swiss-Prot.
01-FEB-1996, sequence version 2.
07-MAR-2006, entry version 61.
Phylogenetic order:
Eukaryota Metazoa Chordata Craniata Vertebrata Euteleostomi Mammalia Eutheria Euarchontoglires Primates Catarrhini Hominidae Homo.
To calculate the pI (Isoelectric point - the pH where a protein has a neutral charge),
go to this page and enter the protein ID (e.g 3MG_ECOLI): http://us.expasy.org/tools/pi_tool.html
Links to references in other databases for protein RFA1_HUMAN:
| Database | Pointer | Add. info#1 | Add. info#2 |
| EMBL | M63488 | AAA36584.1 | - |
| EMBL | AB209732 | BAD92969.1 | ALT_INIT |
| EMBL | AY599563 | AAS94324.1 | - |
| EMBL | BC018126 | AAH18126.1 | - |
| PIR | A40457 | A40457. | |
| PDB | 1EWI | NMR | A=1-114. |
| PDB | 1FGU | X-ray | A/B=182-432. |
| PDB | 1JMC | X-ray | A=181-422. |
| PDB | 1L1O | X-ray | C/F=436-616. |
| PDB | 2B29 | X-ray | A=1-120. |
| PDB | 2B3G | X-ray | A=1-120. |
| IntAct | P27694 | -.1 | |
| Ensembl | ENSG00000132383 | Homo sapiens.1 | |
| H-InvDB | HIX0017624 | -.1 | |
| HGNC | HGNC:10289 | RPA1.1 | |
| MIM | 179835 | gene. | |
| Reactome | P27694 | -.1 | |
| LinkHub | P27694 | -.1 | |
| GO | GO:0005662 | C:DNA replication factor A complex | TAS. |
| GO | GO:0003697 | F:single-stranded DNA binding | TAS. |
| GO | GO:0006310 | P:DNA recombination | TAS. |
| GO | GO:0006281 | P:DNA repair | TAS. |
| GO | GO:0006261 | P:DNA-dependent DNA replication | TAS. |
| InterPro | IPR012340 | OB_NA_bd_sub. | |
| InterPro | IPR004365 | OB_tRNA_NA_bd. | |
| InterPro | IPR007199 | Rep-A_N. | |
| InterPro | IPR004591 | Rpa1. | |
| Pfam | PF04057 | Rep-A_N | 1. |
| Pfam | PF01336 | tRNA_anti | 1. |
| TIGRFAMs | TIGR00617 | rpa1 | 1. |
Keywords:
3D-structure; Direct protein sequencing; DNA replication; DNA-binding; Metal-binding; Nuclear protein; Polymorphism; Zinc; Zinc-finger.
References:
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], AND PARTIAL PROTEIN SEQUENCE.
RX MEDLINE=91268092; PubMed=2050703;
RA Erdile L.F., Heyer W.-D., Kolodner R., Kelly T.J.;
RT "Characterization of a cDNA encoding the 70-kDa single-stranded DNA-
RT binding subunit of human replication protein A and the role of the
RT protein in DNA replication.";
RL J. Biol. Chem. 266:12090-12098(1991).
RN [2]
RP SEQUENCE REVISION TO 217.
RX MEDLINE=93131993; PubMed=8420996;
RA Erdile L.F., Heyer W.-D., Kolodner R., Kelly T.J.;
RT "Type I human complement C2 deficiency. A 28-base pair gene deletion
RT causes skipping of exon 6 during RNA splicing.";
RL J. Biol. Chem. 268:2268-2268(1993).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], AND VARIANT ALA-351.
RC TISSUE=Aortic endothelium;
RA Totoki Y., Toyoda A., Takeda T., Sakaki Y., Tanaka A., Yokoyama S.,
RA Ohara O., Nagase T., Kikuno F.R.;
RL Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND VARIANT ALA-351.
RA Livingston R.J., Rieder M.J., Chung M.-W., Ritchie T.K., Olson A.N.,
RA Nguyen C.P., Nguyen D.A., Poel C.L., Robertson P.D., Schackwitz W.S.,
RA Sherwood J.K., Sherwood A.M., Leithauser B.J., Nickerson D.A.;
RT "NIEHS-SNPs, environmental genome project, NIEHS ES15478, Department
RT of Genome Sciences, Seattle, WA (URL: http://egp.gs.washington.edu).";
RL Submitted (APR-2004) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Uterus;
RX MEDLINE=22388257; PubMed=12477932; DOI=10.1073/pnas.242603899;
RA Strausberg R.L., Feingold E.A., Grouse L.H., Derge J.G.,
RA Klausner R.D., Collins F.S., Wagner L., Shenmen C.M., Schuler G.D.,
RA Altschul S.F., Zeeberg B., Buetow K.H., Schaefer C.F., Bhat N.K.,
RA Hopkins R.F., Jordan H., Moore T., Max S.I., Wang J., Hsieh F.,
RA Diatchenko L., Marusina K., Farmer A.A., Rubin G.M., Hong L.,
RA Stapleton M., Soares M.B., Bonaldo M.F., Casavant T.L., Scheetz T.E.,
RA Brownstein M.J., Usdin T.B., Toshiyuki S., Carninci P., Prange C.,
RA Raha S.S., Loquellano N.A., Peters G.J., Abramson R.D., Mullahy S.J.,
RA Bosak S.A., McEwan P.J., McKernan K.J., Malek J.A., Gunaratne P.H.,
RA Richards S., Worley K.C., Hale S., Garcia A.M., Gay L.J., Hulyk S.W.,
RA Villalon D.K., Muzny D.M., Sodergren E.J., Lu X., Gibbs R.A.,
RA Fahey J., Helton E., Ketteman M., Madan A., Rodrigues S., Sanchez A.,
RA Whiting M., Madan A., Young A.C., Shevchenko Y., Bouffard G.G.,
RA Blakesley R.W., Touchman J.W., Green E.D., Dickson M.C.,
RA Rodriguez A.C., Grimwood J., Schmutz J., Myers R.M.,
RA Butterfield Y.S.N., Krzywinski M.I., Skalska U., Smailus D.E.,
RA Schnerch A., Schein J.E., Jones S.J.M., Marra M.A.;
RT "Generation and initial analysis of more than 15,000 full-length human
RT and mouse cDNA sequences.";
RL Proc. Natl. Acad. Sci. U.S.A. 99:16899-16903(2002).
RN [6]
RP INTERACTION WITH RIP.
RX PubMed=16135809; DOI=10.1128/MCB.25.18.8202-8214.2005;
RA Park J., Seo T., Kim H., Choe J.;
RT "Sumoylation of the novel protein hRIPbeta is involved in replication
RT protein A deposition in PML nuclear bodies.";
RL Mol. Cell. Biol. 25:8202-8214(2005).
RN [7]
RP X-RAY CRYSTALLOGRAPHY (2.4 ANGSTROMS) OF 183-420.
RX MEDLINE=97144356; PubMed=8990123; DOI=10.1038/385176a0;
RA Bochkarev A., Pfuetzner R.A., Edwards A.M., Frappier L.;
RT "Structure of the single-stranded-DNA-binding domain of replication
RT protein A bound to DNA.";
RL Nature 385:176-181(1997).
Feature:
CHAIN 1 616 Replication protein A 70 kDa DNA-binding
subunit.
/FTId=PRO_0000097260.
ZN_FING 481 503 C4-type (Potential).
VARIANT 351 351 T -> A (in dbSNP:5030755).
/FTId=VAR_019236.
TURN 7 8
HELIX 9 16
STRAND 20 20
STRAND 24 33
TURN 36 37
STRAND 38 38
STRAND 41 47
STRAND 49 58
HELIX 60 67
TURN 68 69
STRAND 70 71
TURN 73 74
STRAND 76 86
STRAND 88 90
STRAND 92 103
STRAND 105 105
TURN 106 107
STRAND 108 108
STRAND 110 110
STRAND 113 113
STRAND 116 117
STRAND 184 184
HELIX 187 189
TURN 192 193
STRAND 198 206
STRAND 210 213
STRAND 215 216
STRAND 218 227
STRAND 229 230
STRAND 232 238
HELIX 239 248
TURN 251 252
STRAND 254 258
STRAND 261 264
HELIX 267 269
STRAND 275 279
TURN 282 283
STRAND 285 288
STRAND 291 291
STRAND 293 293
STRAND 302 302
HELIX 305 310
STRAND 311 311
TURN 313 314
STRAND 316 326
STRAND 330 334
TURN 335 338
STRAND 339 349
TURN 351 352
STRAND 355 361
HELIX 362 367
TURN 371 372
STRAND 375 384
TURN 386 387
STRAND 388 392
STRAND 395 396
STRAND 398 402
STRAND 405 405
HELIX 406 416
TURN 417 419
STRAND 442 442
HELIX 445 451
TURN 452 454
STRAND 455 458
STRAND 460 471
STRAND 474 474
TURN 475 476
STRAND 477 480
STRAND 483 483
TURN 484 485
STRAND 489 489
STRAND 491 493
TURN 494 496
STRAND 497 500
TURN 501 504
STRAND 505 509
STRAND 512 521
STRAND 523 524
STRAND 526 532
HELIX 533 540
STRAND 541 542
HELIX 544 550
TURN 551 552
STRAND 553 553
HELIX 555 564
TURN 565 567
STRAND 569 577
STRAND 588 596
HELIX 599 615
Comments:
-!- FUNCTION: It participates in a very early step in initiation. RP-A
is a single-stranded DNA-binding protein. Absolutely required for
simian virus 40 DNA replication in vitro.
-!- SUBUNIT: Heterotrimer of 70, 32/30, and 14 kDa chains. The DNA-
binding activity may reside exclusively on the 70 kDa subunit.
Interacts with RIP.
-!- INTERACTION:
P54132:BLM; NbExp=1; IntAct=EBI-621389, EBI-621372;
P04637:TP53; NbExp=1; IntAct=EBI-621389, EBI-366083;
Q14191:WRN; NbExp=4; IntAct=EBI-621389, EBI-368417;
-!- SUBCELLULAR LOCATION: Nucleus.
-!- SIMILARITY: Belongs to the replication factor-A protein 1 family.
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Sequence length: 616
MVGQLSEGAI AAIMQKGDTN IKPILQVINI RPITTGNSPP RYRLLMSDGL NTLSSFMLAT
QLNPLVEEEQ LSSNCVCQIH RFIVNTLKDG RRVVILMELE VLKSAEAVGV KIGNPVPYNE
GLGQPQVAPP APAASPAASS RPQPQNGSSG MGSTVSKAYG ASKTFGKAAG PSLSHTSGGT
QSKVVPIASL TPYQSKWTIC ARVTNKSQIR TWSNSRGEGK LFSLELVDES GEIRATAFNE
QVDKFFPLIE VNKVYYFSKG TLKIANKQFT AVKNDYEMTF NNETSVMPCE DDHHLPTVQF
DFTGIDDLEN KSKDSLVDII GICKSYEDAT KITVRSNNRE VAKRNIYLMD TSGKVVTATL
WGEDADKFDG SRQPVLAIKG ARVSDFGGRS LSVLSSSTII ANPDIPEAYK LRGWFDAEGQ
ALDGVSISDL KSGGVGGSNT NWKTLYEVKS ENLGQGDKPD YFSSVATVVY LRKENCMYQA
CPTQDCNKKV IDQQNGLYRC EKCDTEFPNF KYRMILSVNI ADFQENQWVT CFQESAEAIL
GQNAAYLGEL KDKNEQAFEE VFQNANFRSF IFRVRVKVET YNDESRIKAT VMDVKPVDYR
EYGRRLVMSI RRSALM