|
|
|
|
|
|
Description:
Replication protein A 14 kDa subunit (RP-A) (RF-A) (Replicationfactor-A protein 3) (p14).
Molecular weight: 13569
View which proteins in this organism that is involved with DNA Repair;
classified after biological processes (using data from the GOA project):
DNA repair( GO:0006281 )
Important dates:
01-FEB-1994, integrated into UniProtKB/Swiss-Prot.
01-FEB-1994, sequence version 1.
07-MAR-2006, entry version 49.
Phylogenetic order:
Eukaryota Metazoa Chordata Craniata Vertebrata Euteleostomi Mammalia Eutheria Euarchontoglires Primates Catarrhini Hominidae Homo.
To calculate the pI (Isoelectric point - the pH where a protein has a neutral charge),
go to this page and enter the protein ID (e.g 3MG_ECOLI): http://us.expasy.org/tools/pi_tool.html
Links to references in other databases for protein RFA3_HUMAN:
| Database | Pointer | Add. info#1 | Add. info#2 |
| EMBL | L07493 | AAA58350.1 | - |
| EMBL | BT007320 | AAP35984.1 | - |
| EMBL | DQ003136 | AAX84517.1 | - |
| EMBL | AC004948 | AAQ96878.1 | - |
| EMBL | BC005264 | AAH05264.1 | - |
| EMBL | BC009868 | AAH09868.1 | - |
| PIR | A46008 | A46008. | |
| PDB | 1L1O | X-ray | A/D=1-121. |
| PDB | 1QUQ | X-ray | B/D=1-121. |
| IntAct | P35244 | -.1 | |
| Ensembl | ENSG00000106399 | Homo sapiens.1 | |
| H-InvDB | HIX0017394 | -.1 | |
| HGNC | HGNC:10291 | RPA3.1 | |
| MIM | 179837 | gene. | |
| Reactome | P35244 | -.1 | |
| LinkHub | P35244 | -.1 | |
| GO | GO:0005662 | C:DNA replication factor A complex | TAS. |
| GO | GO:0003697 | F:single-stranded DNA binding | TAS. |
| GO | GO:0006260 | P:DNA replication | TAS. |
| InterPro | IPR012340 | OB_NA_bd_sub. |
Keywords:
3D-structure; DNA replication; Nuclear protein.
References:
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX MEDLINE=93203195; PubMed=8454588;
RA Umbricht C.B., Kelly T.J.;
RT "Cloning, overexpression, and genomic mapping of the 14-kDa subunit of
RT human replication protein A.";
RL J. Biol. Chem. 268:6131-6138(1993).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S.,
RA Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y.,
RA Phelan M., Farmer A.;
RT "Cloning of human full-length CDSs in BD Creator(TM) system donor
RT vector.";
RL Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RA Livingston R.J., Rieder M.J., Shaffer T., Bertucci C., Baier C.N.,
RA Rajkumar N., Willa H.T., Daniels M., Downing T.K., Stanaway I.B.,
RA Nguyen C.P., Gildersleeve H., Cassidy C.M., Johnson E.J.,
RA Swanson J.E., McFarland I., Yool B., Park C., Nickerson D.A.;
RT "NIEHS-SNPs, environmental genome project, NIEHS ES15478, Department
RT of Genome Sciences, Seattle, WA (URL: http://egp.gs.washington.edu).";
RL Submitted (APR-2005) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX MEDLINE=22737999; PubMed=12853948; DOI=10.1038/nature01782;
RA Hillier L.W., Fulton R.S., Fulton L.A., Graves T.A., Pepin K.H.,
RA Wagner-McPherson C., Layman D., Maas J., Jaeger S., Walker R.,
RA Wylie K., Sekhon M., Becker M.C., O'Laughlin M.D., Schaller M.E.,
RA Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Cordes M., Du H.,
RA Sun H., Edwards J., Bradshaw-Cordum H., Ali J., Andrews S., Isak A.,
RA Vanbrunt A., Nguyen C., Du F., Lamar B., Courtney L., Kalicki J.,
RA Ozersky P., Bielicki L., Scott K., Holmes A., Harkins R., Harris A.,
RA Strong C.M., Hou S., Tomlinson C., Dauphin-Kohlberg S.,
RA Kozlowicz-Reilly A., Leonard S., Rohlfing T., Rock S.M.,
RA Tin-Wollam A.-M., Abbott A., Minx P., Maupin R., Strowmatt C.,
RA Latreille P., Miller N., Johnson D., Murray J., Woessner J.P.,
RA Wendl M.C., Yang S.-P., Schultz B.R., Wallis J.W., Spieth J.,
RA Bieri T.A., Nelson J.O., Berkowicz N., Wohldmann P.E., Cook L.L.,
RA Hickenbotham M.T., Eldred J., Williams D., Bedell J.A., Mardis E.R.,
RA Clifton S.W., Chissoe S.L., Marra M.A., Raymond C., Haugen E.,
RA Gillett W., Zhou Y., James R., Phelps K., Iadanoto S., Bubb K.,
RA Simms E., Levy R., Clendenning J., Kaul R., Kent W.J., Furey T.S.,
RA Baertsch R.A., Brent M.R., Keibler E., Flicek P., Bork P., Suyama M.,
RA Bailey J.A., Portnoy M.E., Torrents D., Chinwalla A.T., Gish W.R.,
RA Eddy S.R., McPherson J.D., Olson M.V., Eichler E.E., Green E.D.,
RA Waterston R.H., Wilson R.K.;
RT "The DNA sequence of human chromosome 7.";
RL Nature 424:157-164(2003).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Kidney, and Lung;
RX MEDLINE=22388257; PubMed=12477932; DOI=10.1073/pnas.242603899;
RA Strausberg R.L., Feingold E.A., Grouse L.H., Derge J.G.,
RA Klausner R.D., Collins F.S., Wagner L., Shenmen C.M., Schuler G.D.,
RA Altschul S.F., Zeeberg B., Buetow K.H., Schaefer C.F., Bhat N.K.,
RA Hopkins R.F., Jordan H., Moore T., Max S.I., Wang J., Hsieh F.,
RA Diatchenko L., Marusina K., Farmer A.A., Rubin G.M., Hong L.,
RA Stapleton M., Soares M.B., Bonaldo M.F., Casavant T.L., Scheetz T.E.,
RA Brownstein M.J., Usdin T.B., Toshiyuki S., Carninci P., Prange C.,
RA Raha S.S., Loquellano N.A., Peters G.J., Abramson R.D., Mullahy S.J.,
RA Bosak S.A., McEwan P.J., McKernan K.J., Malek J.A., Gunaratne P.H.,
RA Richards S., Worley K.C., Hale S., Garcia A.M., Gay L.J., Hulyk S.W.,
RA Villalon D.K., Muzny D.M., Sodergren E.J., Lu X., Gibbs R.A.,
RA Fahey J., Helton E., Ketteman M., Madan A., Rodrigues S., Sanchez A.,
RA Whiting M., Madan A., Young A.C., Shevchenko Y., Bouffard G.G.,
RA Blakesley R.W., Touchman J.W., Green E.D., Dickson M.C.,
RA Rodriguez A.C., Grimwood J., Schmutz J., Myers R.M.,
RA Butterfield Y.S.N., Krzywinski M.I., Skalska U., Smailus D.E.,
RA Schnerch A., Schein J.E., Jones S.J.M., Marra M.A.;
RT "Generation and initial analysis of more than 15,000 full-length human
RT and mouse cDNA sequences.";
RL Proc. Natl. Acad. Sci. U.S.A. 99:16899-16903(2002).
RN [6]
RP X-RAY CRYSTALLOGRAPHY (2.8 ANGSTROMS) IN COMPLEX WITH RPA1 AND RPA2.
RX PubMed=11927569; DOI=10.1093/emboj/21.7.1855;
RA Bochkareva E., Korolev S., Lees-Miller S.P., Bochkarev A.;
RT "Structure of the RPA trimerization core and its role in the multistep
RT DNA-binding mechanism of RPA.";
RL EMBO J. 21:1855-1863(2002).
Feature:
CHAIN 1 121 Replication protein A 14 kDa subunit.
/FTId=PRO_0000097276.
TURN 5 6
STRAND 7 7
STRAND 10 12
HELIX 14 19
STRAND 20 20
TURN 21 22
STRAND 23 34
TURN 36 37
STRAND 38 44
TURN 46 47
STRAND 48 48
STRAND 50 54
STRAND 56 57
STRAND 60 61
STRAND 65 73
TURN 75 76
STRAND 77 77
STRAND 79 86
STRAND 91 92
HELIX 96 108
HELIX 110 112
STRAND 113 113
Comments:
-!- FUNCTION: Required for DNA recombination, repair and replication.
The activity of RP-A is mediated by single-stranded DNA binding
and protein interactions.
-!- SUBUNIT: Heterotrimer of 70, 32/30, and 14 kDa chains. The DNA-
binding activity may reside exclusively on the 70 kDa subunit.
-!- SUBCELLULAR LOCATION: Nucleus.
-----------------------------------------------------------------------
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms
Distributed under the Creative Commons Attribution-NoDerivs License
-----------------------------------------------------------------------
Sequence length: 121
MVDMMDLPRS RINAGMLAQF IDKPVCFVGR LEKIHPTGKM FILSDGEGKN GTIELMEPLD
EEISGIVEVV GRVTAKATIL CTSYVQFKED SHPFDLGLYN EAVKIIHDFP QFYPLGIVQH
D