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Description:
Activator 1 36 kDa subunit (Replication factor C 36 kDa subunit) (A136 kDa subunit) (RF-C 36 kDa subunit) (RFC36) (Replication factor Csubunit 5).
Molecular weight: 38497
View which proteins in this organism that is involved with DNA Repair;
classified after biological processes (using data from the GOA project):
DNA repair( GO:0006281 )
Important dates:
01-FEB-1995, integrated into UniProtKB/Swiss-Prot.
01-FEB-1995, sequence version 1.
07-MAR-2006, entry version 49.
Phylogenetic order:
Eukaryota Metazoa Chordata Craniata Vertebrata Euteleostomi Mammalia Eutheria Euarchontoglires Primates Catarrhini Hominidae Homo.
To calculate the pI (Isoelectric point - the pH where a protein has a neutral charge),
go to this page and enter the protein ID (e.g 3MG_ECOLI): http://us.expasy.org/tools/pi_tool.html
Links to references in other databases for protein RFC5_HUMAN:
| Database | Pointer | Add. info#1 | Add. info#2 |
| EMBL | L07540 | AAB09784.1 | - |
| EMBL | AY254323 | AAO63493.1 | - |
| EMBL | BC001866 | AAH01866.1 | - |
| EMBL | BC013961 | AAH13961.1 | - |
| PDB | 1LFS | Model | A=1-340. |
| IntAct | P40937 | -.1 | |
| Ensembl | ENSG00000111445 | Homo sapiens.1 | |
| H-InvDB | HIX0022829 | -.1 | |
| HGNC | HGNC:9973 | RFC5.1 | |
| MIM | 600407 | gene. | |
| Reactome | P40937 | -.1 | |
| GO | GO:0005660 | C:delta-DNA polymerase cofactor complex | NAS. |
| GO | GO:0019899 | F:enzyme binding | NAS. |
| GO | GO:0006281 | P:DNA repair | NAS. |
| GO | GO:0006260 | P:DNA replication | NAS. |
| InterPro | IPR003593 | AAA_ATPase. | |
| InterPro | IPR003959 | AAA_ATPase_centr. | |
| InterPro | IPR000862 | RFC. | |
| Pfam | PF00004 | AAA | 1. |
| SMART | SM00382 | AAA | 1. |
Keywords:
3D-structure; ATP-binding; DNA replication; Nuclear protein; Nucleotide-binding; Polymorphism.
References:
RN [1]
RP NUCLEOTIDE SEQUENCE.
RX MEDLINE=93181160; PubMed=8441605;
RA O'Donnell M., Onrust R., Dean F.B., Chen M., Hurwitz J.;
RT "Homology in accessory proteins of replicative polymerases -- E. coli
RT to humans.";
RL Nucleic Acids Res. 21:1-3(1993).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND VARIANT THR-13.
RA Rieder M.J., Livingston R.J., Daniels M.R., Chung M.-W.,
RA Miyamoto K.E., Nguyen C.P., Nguyen D.A., Poel C.L., Robertson P.D.,
RA Schackwitz W.S., Sherwood J.K., Witrak L.A., Nickerson D.A.;
RT "NIEHS-SNPs, environmental genome project, NIEHS ES15478, Department
RT of Genome Sciences, Seattle, WA (URL: http://egp.gs.washington.edu).";
RL Submitted (MAR-2003) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Muscle, and Skin;
RX MEDLINE=22388257; PubMed=12477932; DOI=10.1073/pnas.242603899;
RA Strausberg R.L., Feingold E.A., Grouse L.H., Derge J.G.,
RA Klausner R.D., Collins F.S., Wagner L., Shenmen C.M., Schuler G.D.,
RA Altschul S.F., Zeeberg B., Buetow K.H., Schaefer C.F., Bhat N.K.,
RA Hopkins R.F., Jordan H., Moore T., Max S.I., Wang J., Hsieh F.,
RA Diatchenko L., Marusina K., Farmer A.A., Rubin G.M., Hong L.,
RA Stapleton M., Soares M.B., Bonaldo M.F., Casavant T.L., Scheetz T.E.,
RA Brownstein M.J., Usdin T.B., Toshiyuki S., Carninci P., Prange C.,
RA Raha S.S., Loquellano N.A., Peters G.J., Abramson R.D., Mullahy S.J.,
RA Bosak S.A., McEwan P.J., McKernan K.J., Malek J.A., Gunaratne P.H.,
RA Richards S., Worley K.C., Hale S., Garcia A.M., Gay L.J., Hulyk S.W.,
RA Villalon D.K., Muzny D.M., Sodergren E.J., Lu X., Gibbs R.A.,
RA Fahey J., Helton E., Ketteman M., Madan A., Rodrigues S., Sanchez A.,
RA Whiting M., Madan A., Young A.C., Shevchenko Y., Bouffard G.G.,
RA Blakesley R.W., Touchman J.W., Green E.D., Dickson M.C.,
RA Rodriguez A.C., Grimwood J., Schmutz J., Myers R.M.,
RA Butterfield Y.S.N., Krzywinski M.I., Skalska U., Smailus D.E.,
RA Schnerch A., Schein J.E., Jones S.J.M., Marra M.A.;
RT "Generation and initial analysis of more than 15,000 full-length human
RT and mouse cDNA sequences.";
RL Proc. Natl. Acad. Sci. U.S.A. 99:16899-16903(2002).
RN [4]
RP FUNCTION, AND INTERACTION WITH PCNA.
RX MEDLINE=97153138; PubMed=8999859; DOI=10.1074/jbc.272.3.1769;
RA Mossi R., Jonsson Z.O., Allen B.L., Hardin S.H., Huebscher U.;
RT "Replication factor C interacts with the C-terminal side of
RT proliferating cell nuclear antigen.";
RL J. Biol. Chem. 272:1769-1776(1997).
RN [5]
RP INTERACTION WITH RAD17.
RX MEDLINE=21457323; PubMed=11572977; DOI=10.1073/pnas.201373498;
RA Lindsey-Boltz L.A., Bermudez V.P., Hurwitz J., Sancar A.;
RT "Purification and characterization of human DNA damage checkpoint Rad
RT complexes.";
RL Proc. Natl. Acad. Sci. U.S.A. 98:11236-11241(2001).
Feature:
CHAIN 1 340 Activator 1 36 kDa subunit.
/FTId=PRO_0000121751.
NP_BIND 60 67 ATP (Potential).
VARIANT 13 13 A -> T.
/FTId=VAR_018749.
STRAND 6 8
HELIX 13 19
STRAND 20 20
HELIX 22 26
STRAND 27 27
STRAND 30 30
TURN 31 33
STRAND 36 36
HELIX 38 50
TURN 51 51
STRAND 54 60
TURN 63 64
STRAND 65 66
HELIX 67 87
STRAND 88 94
HELIX 96 100
TURN 101 102
HELIX 103 111
STRAND 112 112
HELIX 115 117
STRAND 119 119
STRAND 121 126
TURN 127 127
HELIX 128 130
STRAND 131 131
HELIX 133 145
TURN 146 149
STRAND 150 155
STRAND 157 157
HELIX 159 161
STRAND 162 162
HELIX 164 168
TURN 169 169
STRAND 170 174
TURN 180 181
HELIX 182 194
TURN 195 195
STRAND 197 198
HELIX 200 210
STRAND 211 212
HELIX 214 227
STRAND 229 231
HELIX 233 240
HELIX 245 257
TURN 258 258
HELIX 260 273
STRAND 274 274
HELIX 278 290
TURN 291 291
STRAND 292 294
HELIX 296 313
TURN 314 316
HELIX 319 334
Comments:
-!- FUNCTION: The elongation of primed DNA templates by DNA polymerase
delta and epsilon requires the action of the accessory proteins
proliferating cell nuclear antigen (PCNA) and activator 1.
-!- SUBUNIT: Heterotetramer of subunits of 40, 38, 37, and 36.5 kDa
that can form a complex either with a fifth 140/145 kDa unit, or
with RAD17. The former interacts with PCNA in the presence of ATP,
while the latter has ATPase activity but is not stimulated by
PCNA.
-!- SUBCELLULAR LOCATION: Nucleus (Probable).
-!- SIMILARITY: Belongs to the activator 1 small subunits family.
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Sequence length: 340
METSALKQQE QPAATKIRNL PWVEKYRPQT LNDLISHQDI LSTIQKFINE DRLPHLLLYG
PPGTGKTSTI LACAKQLYKD KEFGSMVLEL NASDDRGIDI IRGPILSFAS TRTIFKKGFK
LVILDEADAM TQDAQNALRR VIEKFTENTR FCLICNYLSK IIPALQSRCT RFRFGPLTPE
LMVPRLEHVV EEEKVDISED GMKALVTLSS GDMRRALNIL QSTNMAFGKV TEETVYTCTG
HPLKSDIANI LDWMLNQDFT TAYRNITELK TLKGLALHDI LTEIHLFVHR VDFPSSVRIH
LLTKMADIEY RLSVGTNEKI QLSSLIAAFQ VTRDLIVAEA