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Description:
G/T mismatch-specific thymine DNA glycosylase (EC 3.2.2.-).
Molecular weight: 46053
View which proteins in this organism that is involved with DNA Repair;
classified after biological processes (using data from the GOA project):
DNA repair( GO:0006281 ) base-excision repair( GO:0006284 )
Important dates:
15-DEC-1998, integrated into UniProtKB/Swiss-Prot.
13-APR-2004, sequence version 2.
07-MAR-2006, entry version 40.
Phylogenetic order:
Eukaryota Metazoa Chordata Craniata Vertebrata Euteleostomi Mammalia Eutheria Euarchontoglires Primates Catarrhini Hominidae Homo.
To calculate the pI (Isoelectric point - the pH where a protein has a neutral charge),
go to this page and enter the protein ID (e.g 3MG_ECOLI): http://us.expasy.org/tools/pi_tool.html
Links to references in other databases for protein TDG_HUMAN:
| Database | Pointer | Add. info#1 | Add. info#2 |
| EMBL | U51166 | AAC50540.1 | - |
| EMBL | AF545435 | AAN16399.1 | - |
| EMBL | BC037557 | AAH37557.1 | - |
| PDB | 1WYW | X-ray | A=112-339. |
| IntAct | Q13569 | -.1 | |
| HGNC | HGNC:11700 | TDG.1 | |
| MIM | 601423 | gene. | |
| Reactome | Q13569 | -.1 | |
| LinkHub | Q13569 | -.1 | |
| GO | GO:0005654 | C:nucleoplasm | TAS. |
| GO | GO:0003684 | F:damaged DNA binding | TAS. |
| GO | GO:0005515 | F:protein binding | IPI. |
| GO | GO:0008263 | F:pyrimidine-specific mismatch base pair DNA ... | TAS. |
| GO | GO:0006284 | P:base-excision repair | TAS. |
| InterPro | IPR003310 | G:TDNA_glycsylse. | |
| InterPro | IPR005122 | UDNA_glycsylseSF. | |
| Pfam | PF03167 | UDG | 1. |
| TIGRFAMs | TIGR00584 | mug | 1. |
Keywords:
3D-structure; Direct protein sequencing; DNA damage; DNA repair; Glycosidase; Hydrolase; Nuclear protein; Polymorphism.
References:
RN [1]
RP NUCLEOTIDE SEQUENCE, AND PARTIAL PROTEIN SEQUENCE.
RX MEDLINE=96278662; PubMed=8662714; DOI=10.1074/jbc.271.22.12767;
RA Neddermann P., Gallinari P., Lettieri T., Schmid D., Truong O.,
RA Hsuan J.J., Wiebauer K., Jiricny J.;
RT "Cloning and expression of human G/T mismatch-specific thymine-DNA
RT glycosylase.";
RL J. Biol. Chem. 271:12767-12774(1996).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND VARIANTS SER-199 AND MET-367.
RA Rieder M.J., Livingston R.J., Daniels M.R., Montoya M.A., Chung M.-W.,
RA Miyamoto K.E., Nguyen C.P., Nguyen D.A., Poel C.L., Robertson P.D.,
RA Schackwitz W.S., Sherwood J.K., Witrak L.A., Nickerson D.A.;
RT "NIEHS-SNPs, environmental genome project, NIEHS ES15478, Department
RT of Genome Sciences, Seattle, WA (URL: http://egp.gs.washington.edu).";
RL Submitted (SEP-2002) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Colon;
RX MEDLINE=22388257; PubMed=12477932; DOI=10.1073/pnas.242603899;
RA Strausberg R.L., Feingold E.A., Grouse L.H., Derge J.G.,
RA Klausner R.D., Collins F.S., Wagner L., Shenmen C.M., Schuler G.D.,
RA Altschul S.F., Zeeberg B., Buetow K.H., Schaefer C.F., Bhat N.K.,
RA Hopkins R.F., Jordan H., Moore T., Max S.I., Wang J., Hsieh F.,
RA Diatchenko L., Marusina K., Farmer A.A., Rubin G.M., Hong L.,
RA Stapleton M., Soares M.B., Bonaldo M.F., Casavant T.L., Scheetz T.E.,
RA Brownstein M.J., Usdin T.B., Toshiyuki S., Carninci P., Prange C.,
RA Raha S.S., Loquellano N.A., Peters G.J., Abramson R.D., Mullahy S.J.,
RA Bosak S.A., McEwan P.J., McKernan K.J., Malek J.A., Gunaratne P.H.,
RA Richards S., Worley K.C., Hale S., Garcia A.M., Gay L.J., Hulyk S.W.,
RA Villalon D.K., Muzny D.M., Sodergren E.J., Lu X., Gibbs R.A.,
RA Fahey J., Helton E., Ketteman M., Madan A., Rodrigues S., Sanchez A.,
RA Whiting M., Madan A., Young A.C., Shevchenko Y., Bouffard G.G.,
RA Blakesley R.W., Touchman J.W., Green E.D., Dickson M.C.,
RA Rodriguez A.C., Grimwood J., Schmutz J., Myers R.M.,
RA Butterfield Y.S.N., Krzywinski M.I., Skalska U., Smailus D.E.,
RA Schnerch A., Schein J.E., Jones S.J.M., Marra M.A.;
RT "Generation and initial analysis of more than 15,000 full-length human
RT and mouse cDNA sequences.";
RL Proc. Natl. Acad. Sci. U.S.A. 99:16899-16903(2002).
RN [4]
RP CHARACTERIZATION.
RX MEDLINE=94173886; PubMed=8127859;
RA Neddermann P., Jiricny J.;
RT "Efficient removal of uracil from G.U mispairs by the mismatch-
RT specific thymine DNA glycosylase from HeLa cells.";
RL Proc. Natl. Acad. Sci. U.S.A. 91:1642-1646(1994).
RN [5]
RP CHARACTERIZATION.
RX MEDLINE=94012674; PubMed=8407958;
RA Neddermann P., Jiricny J.;
RT "The purification of a mismatch-specific thymine-DNA glycosylase from
RT HeLa cells.";
RL J. Biol. Chem. 268:21218-21224(1993).
Feature:
CHAIN 1 410 G/T mismatch-specific thymine DNA
glycosylase.
/FTId=PRO_0000185777.
VARIANT 199 199 G -> S.
/FTId=VAR_018892.
VARIANT 367 367 V -> M.
/FTId=VAR_018893.
CONFLICT 91 91 S -> P (in Ref. 1).
CONFLICT 268 268 V -> G (in Ref. 3).
HELIX 119 121
STRAND 127 127
STRAND 130 131
STRAND 133 140
HELIX 143 148
TURN 149 149
STRAND 150 152
STRAND 154 157
HELIX 159 165
TURN 166 167
STRAND 168 171
HELIX 175 185
STRAND 186 192
STRAND 195 195
STRAND 198 198
HELIX 200 202
HELIX 205 222
STRAND 225 231
HELIX 232 241
TURN 242 243
STRAND 248 249
STRAND 252 254
STRAND 256 256
STRAND 259 259
TURN 260 261
STRAND 262 262
STRAND 265 269
STRAND 273 273
TURN 274 275
STRAND 277 278
STRAND 281 281
HELIX 282 284
TURN 285 285
HELIX 286 299
TURN 300 301
TURN 306 307
STRAND 308 314
HELIX 317 329
STRAND 330 330
Comments:
-!- FUNCTION: In the DNA of higher eukaryotes, hydrolytic deamination
of 5-methylcytosine to thymine leads to the formation of G/T
mismatches. This enzyme corrects G/T mispairs to G/C pairs. It is
capable of hydrolyzing the carbon-nitrogen bond between the sugar-
phosphate backbone of the DNA and a mispaired thymine. In addition
to the G/T, it can remove thymine also from C/T and T/T mispairs
in the order G/T >> C/T > T/T. It has no detectable activity on
apyrimidinic sites and does not catalyze the removal of thymine
from A/T pairs or from single-stranded DNA. It can also remove
uracil and 5-bromouracil from mispairs with guanine.
-!- INTERACTION:
Q13838:BAT1; NbExp=1; IntAct=EBI-348333, EBI-348622;
-!- SUBCELLULAR LOCATION: Nucleus.
-!- SIMILARITY: Belongs to the TDG/mug DNA glycosylase family.
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Sequence length: 410
MEAENAGSYS LQQAQAFYTF PFQQLMAEAP NMAVVNEQQM PEEVPAPAPA QEPVQEAPKG
RKRKPRTTEP KQPVEPKKPV ESKKSGKSAK SKEKQEKITD TFKVKRKVDR FNGVSEAELL
TKTLPDILTF NLDIVIIGIN PGLMAAYKGH HYPGPGNHFW KCLFMSGLSE VQLNHMDDHT
LPGKYGIGFT NMVERTTPGS KDLSSKEFRE GGRILVQKLQ KYQPRIAVFN GKCIYEIFSK
EVFGVKVKNL EFGLQPHKIP DTETLCYVMP SSSARCAQFP RAQDKVHYYI KLKDLRDQLK
GIERNMDVQE VQYTFDLQLA QEDAKKMAVK EEKYDPGYEA AYGGAYGENP CSSEPCGFSS
NGLIESVELR GESAFSGIPN GQWMTQSFTD QIPSFSNHCG TQEQEEESHA