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Description:
Endonuclease III (EC 4.2.99.18) (DNA-(apurinic or apyrimidinic site)lyase).
Molecular weight: 24074
View which proteins in this organism that is involved with DNA Repair;
classified after biological processes (using data from the GOA project):
DNA repair( GO:0006281 ) base-excision repair( GO:0006284 )
Important dates:
08-NOV-2002, integrated into UniProtKB/Swiss-Prot.
01-OCT-2002, sequence version 1.
07-MAR-2006, entry version 29.
Phylogenetic order:
Bacteria Proteobacteria Gammaproteobacteria Enterobacteriales Enterobacteriaceae Buchnera.
To calculate the pI (Isoelectric point - the pH where a protein has a neutral charge),
go to this page and enter the protein ID (e.g 3MG_ECOLI): http://us.expasy.org/tools/pi_tool.html
Links to references in other databases for protein END3_BUCAP:
| Database | Pointer | Add. info#1 | Add. info#2 |
| EMBL | AE014086 | AAM67680.1 | - |
| HSSP | P20625 | 2ABK | |
| GenomeReviews | AE013218_GR | BUsg111.1 | |
| InterPro | IPR003265 | Endo_3c. | |
| InterPro | IPR004035 | EndoIII_FCL. | |
| InterPro | IPR004036 | EndoIII_HhH. | |
| InterPro | IPR000445 | HhH. | |
| InterPro | IPR005759 | Nth. | |
| Pfam | PF00633 | HHH | 1. |
| Pfam | PF00730 | HhH-GPD | 1. |
| SMART | SM00478 | ENDO3c | 1. |
| TIGRFAMs | TIGR01083 | nth | 1. |
| PROSITE | PS00764 | ENDONUCLEASE_III_1 | FALSE_NEG. |
| PROSITE | PS01155 | ENDONUCLEASE_III_2 | 1. |
Keywords:
4Fe-4S; Complete proteome; DNA damage; DNA repair; Glycosidase; Hydrolase; Iron; Iron-sulfur; Lyase; Metal-binding; Multifunctional enzyme.
References:
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX MEDLINE=22084549; PubMed=12089438; DOI=10.1126/science.1071278;
RA Tamas I., Klasson L., Canbaeck B., Naeslund A.K., Eriksson A.-S.,
RA Wernegreen J.J., Sandstroem J.P., Moran N.A., Andersson S.G.E.;
RT "50 million years of genomic stasis in endosymbiotic bacteria.";
RL Science 296:2376-2379(2002).
Feature:
CHAIN 1 209 Endonuclease III.
/FTId=PRO_0000102215.
METAL 187 187 Iron-sulfur (4Fe-4S) (By similarity).
METAL 194 194 Iron-sulfur (4Fe-4S) (By similarity).
METAL 197 197 Iron-sulfur (4Fe-4S) (By similarity).
METAL 203 203 Iron-sulfur (4Fe-4S) (By similarity).
Comments:
-!- FUNCTION: Has both an apurinic and/or apyrimidinic endonuclease
activity and a DNA N-glycosylase activity. Incises damaged DNA at
cytosines, thymines and guanines. Acts on a damaged strand, 5'
from the damaged site (By similarity).
-!- CATALYTIC ACTIVITY: The C-O-P bond 3' to the apurinic or
apyrimidinic site in DNA is broken by a beta-elimination reaction,
leaving a 3'-terminal unsaturated sugar and a product with a
terminal 5'-phosphate.
-!- COFACTOR: Binds 1 4Fe-4S cluster which is not important for the
catalytic activity, but which is probably involved in the proper
positioning of the enzyme along the DNA strand (By similarity).
-!- SIMILARITY: Belongs to the nth/mutY family.
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Sequence length: 209
MNKKKRFEIL SLFYKKNSNP KIELVFSSDF ELLLSVILSA KSTDVMVNKI TGTLFQIANT
PQSILKLGFN KLRHYIKSIG LYNTKSLNII NSAYLIKTKY NNKVPSNRTE LESLPGVGRK
TANIILNVLF NKNTIAVDTH VFRVANRTGF AKGKNVIEVE KKMIKIVPSI FKKYVHFWFV
LHGRYVCTAR QLKCKTCFIE KLCEFDKKK